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1. (WO2018158142) METHOD FOR CELL LINE DEVELOPMENT

Pub. No.:    WO/2018/158142    International Application No.:    PCT/EP2018/054466
Publication Date: Sat Sep 08 01:59:59 CEST 2018 International Filing Date: Sat Feb 24 00:59:59 CET 2018
IPC: C12N 15/67
C12P 21/00
Applicants: GE HEALTHCARE BIO-SCIENCES AB
Inventors: IVANSSON, Daniel
CASTAN, Andreas
Title: METHOD FOR CELL LINE DEVELOPMENT
Abstract:
The present invention relates to an improved method for cell line development (CLD) which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells. The method combines site-directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI) and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts using SDI from that point on.