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1. (WO2018157832) GASTRIC CANCER MONITORING KIT AND USE METHOD THEREFOR

Pub. No.:    WO/2018/157832    International Application No.:    PCT/CN2018/077714
Publication Date: Sat Sep 08 01:59:59 CEST 2018 International Filing Date: Fri Mar 02 00:59:59 CET 2018
IPC: G01N 33/574
C12Q 1/34
Applicants: JIANGSU XIANSIDA BIOTECHNOLOGY CO., LTD.
江苏先思达生物科技有限公司
XIANSIDA NANJING BIOTECHNOLOGY CO., LTD.
先思达(南京)生物科技有限公司
CHANGZHOU JITAI BIOLOGY TECH CO., LTD.
常州吉泰生物科技有限公司
Inventors: CHEN, Cui Ying
陈翠英
Title: GASTRIC CANCER MONITORING KIT AND USE METHOD THEREFOR
Abstract:
Provided by the present invention are a gastric cancer monitoring kit and a use method therefor, the kit comprising: a reagent A, which is prepared by adding 5% of SDS to an ammonium bicarbonate solution having a concentration of 10 mM; a reagent B, which is prepared by adding no less than 2.4 unit/μL of exoglycosidase to NP40 having a concentration of 10%; a reagent C, which is a mixture liquid composed of, in equal volume, a solution of 20 mM of APTS dissolved in 1.2 M of citric acid and a solution of 2M of an organic reducing agent dissolved in DMSO; and a reagent D, which comprises 0.5 μL of NH4AC having a concentration of 100 mM and a pH of 5.6, 0.2 μL of sialidase, and 2.50 μL of hydrogen peroxide. The kit, on the basis of a fingerprint spectrum for detecting oligosaccharide chains in blood glycoprotein serving as a diagnosis index, evaluates a gastric cancer patient, so that a tumor stage of the gastric cancer is diagnosed.