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1. (WO2018157832) GASTRIC CANCER MONITORING KIT AND USE METHOD THEREFOR
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Pub. No.: WO/2018/157832 International Application No.: PCT/CN2018/077714
Publication Date: 07.09.2018 International Filing Date: 01.03.2018
IPC:
G01N 33/574 (2006.01) ,C12Q 1/34 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
574
for cancer
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
34
involving hydrolase
Applicants:
江苏先思达生物科技有限公司 JIANGSU XIANSIDA BIOTECHNOLOGY CO., LTD. [CN/CN]; 中国江苏省泰州市 中国医药城口泰路东侧、新阳路北侧G26幢六层东侧 The 6th Floor of G26 Building, Road North of Xinyanglu, China Pharmaceutical City Taizhou, Jiangsu 225312, CN
先思达(南京)生物科技有限公司 XIANSIDA NANJING BIOTECHNOLOGY CO., LTD. [CN/CN]; 中国江苏省南京市 栖霞区尧化街道甘家边东108号4幢 Room 108 Building 4, Road Gan Jia Bian Dong Lu, Yao Hua Street Qixia District Nanjing, Jiangsu 210046, CN
常州吉泰生物科技有限公司 CHANGZHOU JITAI BIOLOGY TECH CO., LTD. [CN/CN]; 中国江苏省常州市 武进高新技术产业开发区人民东路158号1号楼326室 Room 326 Building 1, Road Renmindonglu 158, Wujin High-tech Industry Park Changzhou, Jiangsu 213161, CN
Inventors:
陈翠英 CHEN, Cui Ying; CN
Agent:
北京德崇智捷知识产权代理有限公司 JW IP LAW FIRM; 中国江苏省南京市 雨花台区西春路1号创智大厦北楼706 706 Room, North of Chuangzhi Building, 1 Xichun Road, Yuhuatai District Nanjing, Jiangsu 210000, CN
Priority Data:
201710120659.402.03.2017CN
Title (EN) GASTRIC CANCER MONITORING KIT AND USE METHOD THEREFOR
(FR) KIT DE SURVEILLANCE DU CANCER GASTRIQUE ET PROCÉDÉ D'UTILISATION ASSOCIÉ
(ZH) 一种胃癌监测试剂盒及其使用方法
Abstract:
(EN) Provided by the present invention are a gastric cancer monitoring kit and a use method therefor, the kit comprising: a reagent A, which is prepared by adding 5% of SDS to an ammonium bicarbonate solution having a concentration of 10 mM; a reagent B, which is prepared by adding no less than 2.4 unit/μL of exoglycosidase to NP40 having a concentration of 10%; a reagent C, which is a mixture liquid composed of, in equal volume, a solution of 20 mM of APTS dissolved in 1.2 M of citric acid and a solution of 2M of an organic reducing agent dissolved in DMSO; and a reagent D, which comprises 0.5 μL of NH4AC having a concentration of 100 mM and a pH of 5.6, 0.2 μL of sialidase, and 2.50 μL of hydrogen peroxide. The kit, on the basis of a fingerprint spectrum for detecting oligosaccharide chains in blood glycoprotein serving as a diagnosis index, evaluates a gastric cancer patient, so that a tumor stage of the gastric cancer is diagnosed.
(FR) La présente invention concerne un kit de surveillance du cancer gastrique et son procédé d'utilisation, le kit comprenant : un réactif A, qui est préparé par ajout de 5 % de SDS à une solution de bicarbonate d'ammonium ayant une concentration de 10 mM ; un réactif B, qui est préparé en ajoutant pas moins de 2,4 unité/µL d'exoglycosidase à NP40 ayant une concentration de 10 % ; un réactif C, qui est un mélange liquide composé, en volume égal, une solution de 20 mM d'APTS dissous dans 1,2 M d'acide citrique et d'une solution de 2M d'un agent réducteur organique dissous dans du DMSO ; et un réactif D, qui comprend 0,5 µL de NH4AC ayant une concentration de 100 mM et un pH de 5,6, 0,2 µL de sialidase, et 2,50 µL de peroxyde d'hydrogène. Le kit, sur la base d'un spectre d'empreintes digitales pour détecter des chaînes oligosaccharidiques dans une glycoprotéine sanguine servant d'index diagnostique, évalue un patient atteint d'un cancer gastrique, de telle sorte qu'un stade tumoral du cancer gastrique est diagnostiqué.
(ZH) 本发明提供了一种胃癌监测试剂盒及其使用方法,该试剂盒包括:试剂A:浓度为10mM的碳酸氢铵溶液中加入5%的SDS配制而成;试剂B:浓度为10%NP40中加入不少于2.4单位/μL糖苷外切酶配制而成;试剂C:等体积20mM APTS的1.2M柠檬酸和2M有机物还原剂的DMSO的混合液;试剂D:0.5μL浓度为100mM、pH 5.6的NH 4AC,0.2μL的唾液酸酶,2.50μL双氧水。该试剂盒基于检测血液糖蛋白中寡糖链的指纹图谱作为诊断指标对胃癌患者进行了评估,以诊断胃癌的肿瘤分期。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)