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1. (WO2018157831) LUNG CANCER MONITORING KIT AND APPLICATION METHOD THEREOF

Pub. No.:    WO/2018/157831    International Application No.:    PCT/CN2018/077713
Publication Date: Sat Sep 08 01:59:59 CEST 2018 International Filing Date: Fri Mar 02 00:59:59 CET 2018
IPC: G01N 33/574
Applicants: JIANGSU XIANSIDA BIOTECHNOLOGY CO LTD
江苏先思达生物科技有限公司
XIANSIDA NANJING BIOTECHNOLOGY CO LTD
先思达(南京)生物科技有限公司
CHANGZHOU JITAI BIOLOGY TECH CO LTD
常州吉泰生物科技有限公司
Inventors: CHEN, Cuiying
陈翠英
Title: LUNG CANCER MONITORING KIT AND APPLICATION METHOD THEREOF
Abstract:
A lung cancer monitoring kit and an application method thereof. The monitoring kit comprises: a reagent A prepared by adding 5% of SDS into an ammonium bicarbonate solution having a concentration of 10 mM; a reagent B prepared by adding not less than 2.2 unit/μL of an exoglycosidase into NP40 having a concentration of 3.3%; a reagent C comprising a mixed solution of equal volumes of 20 mM of APTS in 1.2 M of a citric acid and 1 M of an organic reducing agent in DMSO; and a reagent D comprising 0.25 μL NH4AC having a concentration of 100 mM and pH 5, 0.2 μL of a neuraminidase, and 2.55 μL hydrogen peroxide. The kit is used to evaluate a lung cancer patient on the basis of a diagnostic indicator of a fingerprinting pattern generated by detecting oligosaccharide chains in a blood glycoprotein, so as to diagnose a tumor stage of a lung cancer, implementing regular and non-invasive detection for a lung cancer patient, and monitoring the progress of the disease in real time.