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1. (WO2018148511) A MODULAR UNIVERSAL PLASMID DESIGN STRATEGY FOR THE ASSEMBLY AND EDITING OF MULTIPLE DNA CONSTRUCTS FOR MULTIPLE HOSTS

Pub. No.:    WO/2018/148511    International Application No.:    PCT/US2018/017573
Publication Date: Fri Aug 17 01:59:59 CEST 2018 International Filing Date: Sat Feb 10 00:59:59 CET 2018
IPC: C12N 15/09
C12N 15/10
C12N 15/113
C12N 15/63
C12N 15/64
C12N 15/66
Applicants: ZYMERGEN INC.
Inventors: CHAIKIND, Brian
VAN ROSSUM, Hendrik Marinus
Title: A MODULAR UNIVERSAL PLASMID DESIGN STRATEGY FOR THE ASSEMBLY AND EDITING OF MULTIPLE DNA CONSTRUCTS FOR MULTIPLE HOSTS
Abstract:
The disclosure describes methods, compositions, and kits for high throughput DNA assembly reactions in vitro. The disclosure further describes modular CRISPR DNA constructs comprising modular insert DNA parts flanked by cloning tag segments comprising pre-validated CRISPR protospacer/protospacer adjacent motif sequence combinations.