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1. (WO2018146678) GLYCOALKALOID METABOLISM ENYZYMES (GAMES) AND USES THEREOF

Pub. No.:    WO/2018/146678    International Application No.:    PCT/IL2018/050142
Publication Date: Fri Aug 17 01:59:59 CEST 2018 International Filing Date: Fri Feb 09 00:59:59 CET 2018
IPC: C12N 15/82
Applicants: YEDA RESEARCH AND DEVELOPMENT CO. LTD.
YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM LTD.
Inventors: AHARONI, Asaph
SONAWANE, Prashant
CARDENAS, Pablo D.
BURDMAN, Saul
Title: GLYCOALKALOID METABOLISM ENYZYMES (GAMES) AND USES THEREOF
Abstract:
Disclosed herein are genetically modified plants having altered biological activity of 3- β-hydroxysteroid dehydrogenase/isomerase (GAME25), or 2-oxoglutarate-dependent dioxygenase (GAME31), or a combination thereof, wherein the genetically modified plants have an altered content of at least one cholesterol derived compound selected from the group including a steroidal alkaloid or a glycosylated derivative thereof and an unsaturated or saturated steroidal saponin or a glycoside derivative thereof. Further disclosed herein are genetically modified plants having altered expression of a gene encoding a 3-β-hydroxysteroid dehydrogenase/isomerase (GAME25), or a 2- oxoglutarate-dependent dioxygenase (GAME31), or a combination thereof, wherein the genetically modified plant has an altered content of at least one cholesterol derived compound selected from the group including a steroidal alkaloid or a glycosylated derivative thereof and an unsaturated or saturated steroidal saponin or a glycoside derivative thereof. Methods of producing these genetically modified plants are also disclosed.