The effect of the leaves water extract of lagenaria siceraria (sweet variety) on wound healing and diabetic wound healing in Wistar albino rats was determined in comparison with Fucidin ointment 2% and Solcoseryl ointment 10% (reference ointment used in wound healing ) using excision wound healing model. In the first group (-ve control), the healing took about 12 days to be complemented whereas in the group treated with polyethylene glycol ointment 2% (+ve control) the healing was completed within 11 days. In the group treated with polyethylene glycol containing L. s. aqueous extract 2%), the period of healing was diminished to 7 days whereas in group treated with Fucidin 2 % and Solcoseryl ointment 10% the healing was completed within 10 and 9 days respectively, In diabetic wound healing trial using two groups (treated and untreated group), In untreated group (Group 1) the healing was completed within 14 days with the presence of pus cells during healing period whereas in the treated group, the healing period declined to 9 days to demonstrate intelligible effect of L. s. leaves aqueous extract on diabetic wound healing. In the clinical trial all the treated volunteers (about 42) had been recovered, 100 % results was obtained. Some of the patients recovered within three days, others within 7 days whereas others take 10-15 days. In acute oral toxicity, Lagenaria siceraria /eaves water extract didn't show any signs of toxicity during all of the observation period, no mortality or any reduction of weight has been registered during the experimental period. In topical treatment, no irritation, no corrosion or any signs of allergy or skin sensitivity against the ointment. Phytochemical screening of the leaves water extract declared presence of Polyuronide (mucillage), glucides, reducing compounds, saponins and tannins. In testing antimicrobial activity of Lagenaria siceraria leaves water extract against Staphylococcus aureus, Klebsiella pneumonia, Proteus vulgaris, Pseudomonas aeruginosa and Candida albicans, it didn't detect any activity against any of the tested organisms, lac inhibition zone declared zero reading.