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1. (WO2018146484) SCREENING METHOD AND KIT TO DETECT RECEPTOR ACTIVATION
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Pub. No.: WO/2018/146484 International Application No.: PCT/GB2018/050367
Publication Date: 16.08.2018 International Filing Date: 09.02.2018
IPC:
C12N 15/62 (2006.01) ,C12N 9/12 (2006.01) ,C07K 14/245 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
62
DNA sequences coding for fusion proteins
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10
Transferases (2.)
12
transferring phosphorus containing groups, e.g. kinases (2.7)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195
from bacteria
24
from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
245
Escherichia (G)
Applicants:
UNIVERSITY OF PORTSMOUTH HIGHER EDUCATION CORPORATION [GB/GB]; University House Winston Churchill Avenue Portsmouth Hampshire PO1 2UP, GB
Inventors:
DRAHEIM, Roger; GB
Agent:
WITHERS & ROGERS LLP; 4 More London Riverside London London SE1 2AU, GB
ELSY, David; GB
Priority Data:
1702165.009.02.2017GB
Title (EN) SCREENING METHOD AND KIT TO DETECT RECEPTOR ACTIVATION
(FR) PROCÉDÉ DE CRIBLAGE ET KIT POUR DÉTECTER UNE ACTIVATION DE RÉCEPTEUR
Abstract:
(EN) The invention comprises a plasmid comprising: (i) a nucleotide sequence encoding a part of a sensor histidine kinase (SHK) comprising the intracellular domain of the SHK wherein the SHK intracellular domain comprises one or more C-terminus aromatic amino acid, and/or one or more positively charged amino acid substitutions or sequence rearrangements compared to the native SHK intracellular domain sequence and the part of the SHK does not comprise at least a portion of the extracellular or periplasmic domain of the SHK. (ii) a cloning site for the insertion of a nucleotide sequence encoding at least a part of an extracellular or periplasmic domain of a heterologous SHK inframe with the nucleotide sequence encoding the SHK intracellular domain.
(FR) L'invention comprend un plasmide comprenant : (i) une séquence nucléotidique codant pour une partie d'un capteur histidine kinase (SHK) comprenant le domaine intracellulaire de SHK, le domaine intracellulaire de SHK comprenant un ou plusieurs acides aminés aromatiques d'extrémité C-terminale, et/ou une ou plusieurs substitutions d'acides aminés chargées positivement ou un ou plusieurs réarrangements de séquences par rapport à la séquence de domaine intracellulaire de SHK native, et la partie du SHK ne comprend pas au moins une partie du domaine extracellulaire ou périplasmique du SHK ; (ii) un site de clonage pour l'insertion d'une séquence nucléotidique codant pour au moins une partie d'un domaine extracellulaire ou périplasmique d'une intrame de SHK hétérologue avec la séquence nucléotidique codant pour le domaine intracellulaire de SHK.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)