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1. WO2018146067 - PROCESS FOR THE PURIFICATION OF SOLUBLE PSGL-1 PROTEIN VARIANTS

Publication Number WO/2018/146067
Publication Date 16.08.2018
International Application No. PCT/EP2018/052889
International Filing Date 06.02.2018
IPC
C07K 14/47 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
CPC
C07K 14/47
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
C07K 14/70596
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
705Receptors; Cell surface antigens; Cell surface determinants
70596Molecules with a "CD"-designation not provided for elsewhere
C07K 2319/01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
01containing a localisation/targetting motif
Applicants
  • BRACCO SUISSE SA [CH]/[CH]
Inventors
  • MAISANO, Federico
  • CRIVELLIN, Federico
Agents
  • RAVIZZA, Claudio
Priority Data
17155366.209.02.2017EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) PROCESS FOR THE PURIFICATION OF SOLUBLE PSGL-1 PROTEIN VARIANTS
(FR) PROCÉDÉ DE PURIFICATION DE VARIANTS SOLUBLES DE PROTÉINES PSGL-1
Abstract
(EN)
The present invention discloses a method for the purification of highly acidic recombinant proteins. The acidic protein is preferably the extracellular region of PSGL-1 or fusion and/or chimeric proteins comprising this soluble portion. Purification is a three-steps chromatography which comprises separation on an Anion Exchange solid-phase, a Hydrophobic Interaction and a Hydroxyapatite. Purity and yields are optimal and the process can be easily scaled up and automated.
(FR)
La présente invention concerne un procédé de purification de protéines recombinantes hautement acides. La protéine acide est de préférence la région extracellulaire de PSGL-1 ou des protéines de fusion et/ou chimériques comprenant cette partie soluble. La purification est une chromatographie à trois étapes qui comprend la séparation sur une phase solide d'échange d'anions, une interaction hydrophobe et une hydroxyapatite. La pureté et les rendements de l'invention sont optimisés et le processus peut être facilement mis à l'échelle et automatisé.
Also published as
Latest bibliographic data on file with the International Bureau