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1. (WO2018145041) SYSTEMS AND METHODS FOR NUCLEIC ACID PREPARATION
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Pub. No.: WO/2018/145041 International Application No.: PCT/US2018/016926
Publication Date: 09.08.2018 International Filing Date: 05.02.2018
IPC:
C12N 9/22 (2006.01) ,C12N 15/10 (2006.01) ,C12P 19/34 (2006.01) ,C40B 20/04 (2006.01) ,C40B 40/06 (2006.01)
[IPC code unknown for C12N 9/22][IPC code unknown for C12N 15/10][IPC code unknown for C12P 19/34][IPC code unknown for C40B 20/04][IPC code unknown for C40B 40/06]
Applicants:
10X GENOMICS, INC. [US/US]; 7068 Koll Center Parkway Suite 401 Pleasanton, California 94566, US
Inventors:
WHEELER, Tobias Daniel; US
BHARADWAJ, Rajiv; US
PFEIFFER, Katherine; US
Agent:
TIAN, Feng; US
Priority Data:
62/455,12906.02.2017US
Title (EN) SYSTEMS AND METHODS FOR NUCLEIC ACID PREPARATION
(FR) SYSTÈMES ET PROCÉDÉS DE PRÉPARATION D’ACIDES NUCLÉIQUES
Abstract:
(EN) The present disclosure provides methods and systems for nucleic acid processing. A method for preparing a sequencing set may include providing a template nucleic acid and amplifying the template nucleic acid to provide a complementary nucleic acid. Next, the complementary nucleic acid may be fragmented and barcoded to produce a first set of barcoded fragments comprising a plurality of first barcoded fragments. Next, the plurality of first barcoded fragments may be fragmented to yield a second set of barcoded fragments comprising a plurality of second barcoded fragments.
(FR) La présente invention concerne des procédés et des systèmes de traitement d’acide nucléique. Un procédé de préparation d'un ensemble de séquençage permet de produire un acide nucléique gabarit et d'amplifier l'acide nucléique gabarit pour produire un acide nucléique complémentaire. Ensuite, l'acide nucléique complémentaire peut être fragmenté et codé par code à barres pour produire un premier ensemble de fragments à code-barres comprenant une pluralité de premiers fragments à code-barres. Ensuite, la pluralité de premiers fragments à code-barres peut être fragmentée pour produire un second ensemble de fragments à code-barres comprenant une pluralité de seconds fragments à code-barres.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)