Search International and National Patent Collections

1. (WO2018143243) METHOD FOR PRODUCING INDUCED PLURIPOTENT STEM CELLS

Pub. No.:    WO/2018/143243    International Application No.:    PCT/JP2018/003120
Publication Date: Fri Aug 10 01:59:59 CEST 2018 International Filing Date: Thu Feb 01 00:59:59 CET 2018
IPC: C12N 5/10
C12N 5/0783
C12N 5/0786
C12N 15/09
Applicants: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
国立大学法人神戸大学
Inventors: AOI, Takayuki
青井 貴之
Title: METHOD FOR PRODUCING INDUCED PLURIPOTENT STEM CELLS
Abstract:
Provided is a method for producing iPS cells. Specifically, provided is a method for producing iPS cells having a γδ-TCR reconstituted gene. Also provided are cell populations including the produced iPS cells. After collected blood cells are stimulated by IL-2 and bisphosphonate, a cell initiation factor is introduced using a Sendai virus (SeV) vector. This method can effectively produce iPS cells having a γδ-TCR reconstituted gene. In particular, the method need not include a step for treating the blood cells by an antibody prior to the step in which the blood cells are stimulated by bisphosphonate and one or more interleukins selected from IL-2, IL-15, and IL-23. In addition, iPS cells produced by this method can be differentiated into desired cells by differentiation induction treatment.