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1. (WO2018141030) EXPRESSION OF NITROGENASE POLYPEPTIDES IN PLANT CELLS

Pub. No.:    WO/2018/141030    International Application No.:    PCT/AU2018/050084
Publication Date: Fri Aug 10 01:59:59 CEST 2018 International Filing Date: Wed Feb 07 00:59:59 CET 2018
IPC: C12N 5/14
C07K 14/26
C12N 9/02
C12N 15/82
Applicants: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION
Inventors: WOOD, Craig Christopher
ALLEN, Robert Silas
OKADA, Shoko
WARDEN, Andrew Charles
TILBROOK, Kimberley Thelma
TAYLOR, Matthew Craig
Title: EXPRESSION OF NITROGENASE POLYPEPTIDES IN PLANT CELLS
Abstract:
The present invention relates to methods and means for producing nitrogenase polypeptides in the mitochondria of plant cells. The present disclosure provides plant cells that express one or more MTP-Nif fusions and/or translational NifD-NifK and NifE-NifN fusions. The present disclosure also provides nucleic acid constructs encoding these fusions as well as expression constructs for expression and targeting of the fusions to the mitochondria of plant cells. The present disclosure also provides transgenic plants comprising the plant cells of the invention and products obtained therefrom.