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1. (WO2018138385) REPAIR TEMPLATE LINKAGE TO ENDONUCLEASES FOR GENOME ENGINEERING

Pub. No.:    WO/2018/138385    International Application No.:    PCT/EP2018/052313
Publication Date: Fri Aug 03 01:59:59 CEST 2018 International Filing Date: Wed Jan 31 00:59:59 CET 2018
IPC: C12N 15/82
C12N 15/10
C12N 15/11
C12N 15/90
A01H 5/00
Applicants: KWS SAAT SE
Inventors: LABS, Mathias
Title: REPAIR TEMPLATE LINKAGE TO ENDONUCLEASES FOR GENOME ENGINEERING
Abstract:
The present invention relates to artificial molecular complexes comprising at least one site-specific nuclease and directly interacting therewith at least one repair template docking domain, said repair template docking domain interacting with at least one repair template nucleic acid sequence. The artificial complex can further comprise at least one interaction domain. The artificial molecular complexes are configured to mediate repair of a DNA target sequence in a prokaryotic or eukaryotic organism with high precision in a targeted way and can thus be used for genome engineering in a prokaryotic or a eukaryotic cell or organism, or editing of a viral genome. Further provided are methods of modifying at least one DNA target sequence in a prokaryotic or eukaryotic cell or a viral genome, e.g., for trait development, or for treating a disease. Additionally, there is provided a method for manufacturing a plant, plant cell, a plant material, or a derivative, or a progeny thereof comprising or edited by at least one artificial molecular complex.