Search International and National Patent Collections

1. (WO2018136864) ANTIMICROBIC SUSCEPTIBILITY TESTING USING DIGITAL MICROSCOPY

Pub. No.:    WO/2018/136864    International Application No.:    PCT/US2018/014660
Publication Date: Fri Jul 27 01:59:59 CEST 2018 International Filing Date: Tue Jan 23 00:59:59 CET 2018
IPC: C12Q 1/18
C12M 1/34
Applicants: BECKMAN COULTER, INC.
Inventors: SEI, Katherine
LEWIS, David
Title: ANTIMICROBIC SUSCEPTIBILITY TESTING USING DIGITAL MICROSCOPY
Abstract:
An optimized testing method is used to determine minimum inhibitory concentration (MIC) of a particular antimicrobic for use with a particular microbe. The testing method includes iteratively imaging the microbes mixed with various concentrations of the antimicrobic. The images are thereafter processed to determine one or more of the amount/count of the microbes in each image, the total area occupied by the microbes in each image, and the ratio between the area occupied by the microbes and the count of the microbes. Once a sufficient amount of data is collected, the MIC is determined based on one or more of the count, area, and ratio datasets.