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1. WO2018136118 - GENOTYPING BY POLYMERASE BINDING

Publication Number WO/2018/136118
Publication Date 26.07.2018
International Application No. PCT/US2017/051025
International Filing Date 11.09.2017
IPC
C12Q 1/68
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12Q 1/6869
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6869Methods for sequencing
C12Q 1/6874
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6869Methods for sequencing
6874involving nucleic acid arrays, e.g. sequencing by hybridisation
Applicants
  • OMNIOME, INC. [US]/[US]
Inventors
  • DAMBACHER, Corey M.
  • VAN NGUYEN, Michael
Agents
  • THOMAS, Tiffany B.
Priority Data
62/448,63020.01.2017US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) GENOTYPING BY POLYMERASE BINDING
(FR) GÉNOTYPAGE PAR LIAISON PAR POLYMÉRASE
Abstract
(EN)
A method for identifying target alleles, that includes steps of (a) forming a plurality of stabilized ternary complexes at a plurality of features on an array, wherein the stabilized ternary complexes each has a polymerase, a template nucleic acid having a target allele of a locus, a primer hybridized to the locus, and a next correct nucleotide having a cognate in the locus, wherein either (i) the primer is an allele-specific primer having a 3' nucleotide that is a cognate nucleotide for the target allele, or (ii) the primer is a locus-specific primer and the next correct nucleotide hybridizes to the target allele; and (b) detecting stabilized ternary complexes at the features, thereby identifying the target alleles.
(FR)
L'invention concerne un procédé pour identifier des allèles cibles, qui comprend les étapes consistant : (a) à former une pluralité de complexes ternaires stabilisés au niveau d'une pluralité de traits sur un réseau, les complexes ternaires stabilisés présentant chacun une polymérase, un acide nucléique à matrice présentant un allèle cible d'un locus, une amorce étant hybridée au locus et un nucléotide correct suivant présentant un parent dans le locus, (i) l'amorce étant une amorce spécifique d'allèle présentant un nucléotide 3' qui est un nucléotide apparenté pour l'allèle cible ou (ii) l'amorce étant une amorce spécifique de locus et le nucléotide correct suivant s'hybridant à l'allèle cible ; et (b) à détecter des complexes ternaires stabilisés au niveau des traits, ce qui permet d'identifier les allèles cibles.
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