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1. (WO2018135593) CELL PRODUCTION METHOD AND KIT FOR PRODUCING CELLS

Pub. No.:    WO/2018/135593    International Application No.:    PCT/JP2018/001441
Publication Date: Fri Jul 27 01:59:59 CEST 2018 International Filing Date: Fri Jan 19 00:59:59 CET 2018
IPC: C12N 5/00
C12M 3/00
Applicants: AGC INC.
AGC株式会社
Inventors: NAKANISHI Tomoaki
中西 智亮
SHIMOSAKA Takamichi
下坂 鷹典
EGUCHI Hajime
江口 創
OKI Satoshi
沖 聡
Title: CELL PRODUCTION METHOD AND KIT FOR PRODUCING CELLS
Abstract:
Provided is a production method for cells, whereby cells cultured on a cell culture substrate can be more easily recovered. The cell production method has: a culture step in which cells are cultured upon a water repellant layer of the cell culture substrate having a substrate main body and the water repellant layer arranged upon the substrate main body; and a recovery step in which the water repellant layer and a solvent are caused to come in contact and cells are separated and recovered from on the water repellant layer. The cell production method is characterized by the solvent having elastic modulus B/elastic modulus A being no more than 0.2. Elastic modulus B/elastic modulus A is the ratio between: an elastic modulus B for the water repellant layer at the section of contact between the water repellant layer and the solvent, measured when the water repellant layer and the solvent are caused to come in contact; and an elastic modulus A for the water repellant layer in a section of contact between the water repellant layer and phosphate buffered saline measured when the water repellant layer and phosphate buffered saline are caused to come in contact.