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1. (WO2018133513) GENOTOXIC SUBSTANCE DETECTION VECTOR AND DETECTION METHOD THEREOF

Pub. No.:    WO/2018/133513    International Application No.:    PCT/CN2017/110897
Publication Date: Fri Jul 27 01:59:59 CEST 2018 International Filing Date: Wed Nov 15 00:59:59 CET 2017
IPC: C12N 15/70
C12N 1/21
C12Q 1/10
Applicants: SOUTH CHINA UNIVERSITY OF TECHNOLOGY
华南理工大学
Inventors: LI, Shuang
李爽
ZHUO, Min
卓敏
YUAN, Pengfei
袁鹏飞
Title: GENOTOXIC SUBSTANCE DETECTION VECTOR AND DETECTION METHOD THEREOF
Abstract:
A genotoxic substance detection vector and a detection method thereof are provided. The vector is an Escherichia coli expression vector sequentially having, from a 5' end to a 3' end, a genotoxic response promoter, a bacteriophage lysis gene, and an Escherichia coli terminator. The detection method includes introducing the genotoxic substance response vector into Escherichia coli to obtain recombinant bacteria, incubating the recombinant bacteria and genotoxic substances, and lysing the Escherichia coli. The recombinant Escherichia coli has the genotoxic response vector, and a self-cell lysis is initiated when the recombinant bacteria contacts the genotoxic substances. The detection method quantitates genotoxic substances by means of lysis efficiency. The method consumes little time, has a high detection sensitivity, is easy to implement, low cost, and easy to promote.