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|1. (WO2018133513) GENOTOXIC SUBSTANCE DETECTION VECTOR AND DETECTION METHOD THEREOF|
|Applicants:||SOUTH CHINA UNIVERSITY OF TECHNOLOGY
|Title:||GENOTOXIC SUBSTANCE DETECTION VECTOR AND DETECTION METHOD THEREOF|
A genotoxic substance detection vector and a detection method thereof are provided. The vector is an Escherichia coli expression vector sequentially having, from a 5' end to a 3' end, a genotoxic response promoter, a bacteriophage lysis gene, and an Escherichia coli terminator. The detection method includes introducing the genotoxic substance response vector into Escherichia coli to obtain recombinant bacteria, incubating the recombinant bacteria and genotoxic substances, and lysing the Escherichia coli. The recombinant Escherichia coli has the genotoxic response vector, and a self-cell lysis is initiated when the recombinant bacteria contacts the genotoxic substances. The detection method quantitates genotoxic substances by means of lysis efficiency. The method consumes little time, has a high detection sensitivity, is easy to implement, low cost, and easy to promote.