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1. (WO2018132871) METHOD OF PRODUCING CHEMICALS IN YEAST
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2018/132871 International Application No.: PCT/AU2018/050029
Publication Date: 26.07.2018 International Filing Date: 17.01.2018
IPC:
C12N 1/15 (2006.01) ,C12N 1/32 (2006.01) ,C12N 15/81 (2006.01) ,C07C 31/04 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
14
Fungi; Culture media therefor
15
modified by introduction of foreign genetic material
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
32
Processes using, or culture media containing, lower alkanols, i.e. C1 to C6
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
80
for fungi
81
for yeasts
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
C
ACYCLIC OR CARBOCYCLIC COMPOUNDS
31
Saturated compounds having hydroxy or O-metal groups bound to acyclic carbon atoms
02
Monohydroxylic acyclic alcohols
04
Methanol
Applicants: MACQUARIE UNIVERSITY[AU/AU]; Balaclava Road North Ryde, New South Wales 2109, AU
Inventors: WILLIAMS, Thomas; AU
PAULSEN, Ian; AU
Agent: FPA PATENT ATTORNEYS PTY LTD; Level 43 101 Collins Street Melbourne, Victoria 3000, AU
Priority Data:
201790012917.01.2017AU
Title (EN) METHOD OF PRODUCING CHEMICALS IN YEAST
(FR) PROCÉDÉ DE PRODUCTION DE PRODUITS CHIMIQUES DANS UNE LEVURE
Abstract:
(EN) The present invention relates to methods for engineering yeast strains which can be used in the production of industrially important chemicals. Nucleic acids encoding components of the methanol utilization pathway are used to modify yeast cells such as Saccharomyces cerevisiae to enable them to grow in media in which methanol is the only or main energy source. The nucleic acids may encode proteins including alcohol oxidase, dihydroxyacetone synthase, dihydroxyacetone kinase, fructose- 1-6-bisphosphate aldolase, fructose-1-6-bisphosphatase, sedoheptulose bisphosphatase, ribose-5-phosphate isomerase, D-ribulose-5-phosphate-3-epimerase, transketolase 1 or 2, transaldolase and formaldehyde dehydrogenase.
(FR) La présente invention concerne des procédés d'ingénierie de souches de levure qui peuvent être utilisés dans la production de produits chimiques industriellement importants. Des acides nucléiques codant des composants du trajet d'utilisation du méthanol sont utilisés pour modifier des cellules de levure telles que des Saccharomyces cerevisiae pour leur permettre de croître dans des milieux dans lesquels le méthanol est la seule source d'énergie ou la source d'énergie principale. Les acides nucléiques peuvent coder des protéines comprenant de l'alcool oxydase, de la dihydroxyacétone synthase, de la dihydroxyacétone kinase, du fructose-1-6-bisphosphate aldolase, de la fructose-1-6-bisphosphatase, de la sédoheptulose bisphosphatase, ribose-5-phosphate isomérase, D-ribulose-5-phosphate-3-épimérase, transkétolase 1 ou 2, de la transaldolase et de la formaldéhyde déshydrogénase.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)