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1. (WO2018132459) METHODS AND COMPOSITIONS FOR REDUCING REDUNDANT MOLECULAR BARCODES CREATED IN PRIMER EXTENSION REACTIONS

Pub. No.:    WO/2018/132459    International Application No.:    PCT/US2018/013143
Publication Date: Fri Jul 20 01:59:59 CEST 2018 International Filing Date: Thu Jan 11 00:59:59 CET 2018
IPC: C12Q 1/68
C40B 20/04
C40B 70/00
Applicants: PARAGON GENOMICS, INC.
Inventors: LIU, Zhitong
LIU, Guoying
LIU, Juehui
CHEN, Tao
Title: METHODS AND COMPOSITIONS FOR REDUCING REDUNDANT MOLECULAR BARCODES CREATED IN PRIMER EXTENSION REACTIONS
Abstract:
Methods, compositions, systems and kits to introduce a controlled-number of molecular barcodes onto DNA fragments by reducing redundant molecular barcodes formed in template-dependent primer extension reactions, and to find variant frequencies from both strands of DNA The methods, compositions, systems and kits described herein may include, or include the use of, one or more single- stranded DNA specific nucleases that cleave the single-stranded regions containing unmatched base pairs in amplification products.