Some content of this application is unavailable at the moment.
If this situation persist, please contact us atFeedback&Contact
1. (WO2018130212) PHYTASE YKAPPA MUTANT HAVING IMPROVED PEPSIN RESISTANCE AND INCREASED CATALYTIC EFFICIENCY
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2018/130212 International Application No.: PCT/CN2018/072549
Publication Date: 19.07.2018 International Filing Date: 15.01.2018
IPC:
C12N 9/16 (2006.01) ,C12N 15/55 (2006.01) ,C12N 15/70 (2006.01) ,A23K 20/189 (2016.01) ,C12N 1/21 (2006.01) ,C12R 1/19 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
16
acting on ester bonds (3.1)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
52
Genes encoding for enzymes or proenzymes
55
Hydrolases (3)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70
Vectors or expression systems specially adapted for E. coli
[IPC code unknown for A23K 20/189]
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
20
Bacteria; Culture media therefor
21
modified by introduction of foreign genetic material
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
R
INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1
Micro-organisms
01
Bacteria or actinomycetales
185
Escherichia
19
Escherichia coli
Applicants:
牛灿芳 NIU, Canfang [CN/CN]; CN (US)
杨培龙 YANG, Peilong [CN/CN]; CN (US)
姚斌 YAO, Bin [CN/CN]; CN (US)
李阳阳 LI, Yangyang [CN/CN]; CN (US)
于大力 YU, Dali [CN/CN]; CN (US)
罗会颖 LUO, Huiying [CN/CN]; CN (US)
黄火清 HUANG, Huoqing [CN/CN]; CN (US)
王亚茹 WANG, Yaru [CN/CN]; CN (US)
中国农业科学院饲料研究所 FEED RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES [CN/CN]; 中国北京市 中关村南大街12号 No. 12, Zhongguancun Nandajie, Haidian District Beijing 100081, CN
Inventors:
牛灿芳 NIU, Canfang; CN
杨培龙 YANG, Peilong; CN
姚斌 YAO, Bin; CN
李阳阳 LI, Yangyang; CN
于大力 YU, Dali; CN
罗会颖 LUO, Huiying; CN
黄火清 HUANG, Huoqing; CN
王亚茹 WANG, Yaru; CN
Agent:
北京法思腾知识产权代理有限公司 FAST INTELLECTUAL PROPERTY LAW, LLC; 中国北京市 海淀区中关村北二条13号1号楼202高宇 GAO, Yu Room 202 Building 1 No. 13 Zhongguancun Beiertiao, Haidian District Beijing 100190, CN
Priority Data:
201710027075.215.01.2017CN
Title (EN) PHYTASE YKAPPA MUTANT HAVING IMPROVED PEPSIN RESISTANCE AND INCREASED CATALYTIC EFFICIENCY
(FR) MUTANT DE PHYTASE YKAPPA AYANT UNE RÉSISTANCE À LA PEPSINE AMÉLIORÉE ET UNE EFFICACITÉ CATALYTIQUE ACCRUE
(ZH) 胃蛋白抗性改良和催化效率提高的植酸酶YkAPPA突变体
Abstract:
(EN) A phytase YkAPPA mutant having improved pepsin resistance and increased catalytic efficiency. Leucine at position 162 of the phytase amino acid sequence as shown in SEQ ID NO. 1 is mutated to glycine or alanine, or glutamic acid at position 230 is mutated to glycine, alanine, proline, arginine, serine, threonine or aspartic acid.
(FR) L'invention concerne le domaine du génie génétique et, en particulier, un mutant de phytase YkAPPA ayant une résistance à la pepsine améliorée et une efficacité catalytique accrue. La leucine en position 162 de la séquence d'acides aminés de phytase telle que représentée dans SEQ ID NO : 1 est mutée en glycine ou en alanine ou l'acide glutamique en position 230 est muté en glycine, alanine, proline, arginine, sérine, thréonine ou acide aspartique. La résistance à la pepsine du mutant de phytase est considérablement améliorée et l'efficacité catalytique est augmentée jusqu'à 2,1 fois, ce qui est avantageux dans l'industrie de développement d'enzymes d'alimentation économique.
(ZH) 植酸酶突变体胃蛋白抗性改良和催化效率提高的植酸酶YkAPPA突变体。氨基酸序列如SEQ ID NO.1所示的植酸酶的第162位亮氨酸突变为甘氨酸或丙氨酸;或第230位谷氨酸突变为甘氨酸、丙氨酸、脯氨酸、精氨酸、丝氨酸、苏氨酸或天冬氨酸。
front page image
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)