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1. WO2018129644 - METHOD, REACTION SYSTEM AND KIT FOR IMPROVING QUALITY OF NUCLEIC ACID POLYMERIZATION SEQUENCING

Publication Number WO/2018/129644
Publication Date 19.07.2018
International Application No. PCT/CN2017/070711
International Filing Date 10.01.2017
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12Q 1/68
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
Applicants
  • 深圳华大智造科技有限公司 MGI TECH CO., LTD. [CN]/[CN]
Inventors
  • 李计广 LI, Jiguang
  • 马可心 MA, Kexin
  • 邱敏 QIU, Min
  • 陈奥 CHEN, Ao
  • 徐崇钧 XU, Chongjun
  • 章文蔚 ZHANG, Wenwei
Agents
  • 深圳鼎合诚知识产权代理有限公司 DHC IP ATTORNEYS
Priority Data
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) METHOD, REACTION SYSTEM AND KIT FOR IMPROVING QUALITY OF NUCLEIC ACID POLYMERIZATION SEQUENCING
(FR) PROCÉDÉ, SYSTÈME DE RÉACTION ET KIT POUR AMÉLIORER LA QUALITÉ DE SÉQUENÇAGE PAR POLYMÉRISATION D'ACIDE NUCLÉIQUE
(ZH) 一种提高核酸聚合测序质量的方法、反应体系和试剂盒
Abstract
(EN)
Disclosed are a method, a reaction system and a kit for improving the quality of nucleic acid polymerization sequencing. The method for improving the quality of nucleic acid polymerization sequencing of the present application comprises: adding a second nucleotide into a polymerization sequencing reaction solution, a 3' saccharide hydroxyl group of the second nucleotide being provided with a blocking modification but no fluorescence modification.
(FR)
L'invention concerne un procédé, un système de réaction et un kit pour améliorer la qualité de séquençage par polymérisation d'acide nucléique. Le procédé d'amélioration de la qualité de séquençage de polymérisation d'acide nucléique de la présente invention consiste à : ajouter un second nucléotide dans une solution de réaction de séquençage par polymérisation, un groupe hydroxyle du saccharide en 3' du second nucléotide étant doté d'une modification de blocage mais pas d'une modification de fluorescence.
(ZH)
公开了一种提高核酸聚合测序质量的方法、反应体系和试剂盒。本申请的提高核酸聚合测序质量的方法,包括在聚合测序的反应液中加入第二类核苷酸,第二类核苷酸的3'糖羟基具有阻断修饰但没有荧光修饰。
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Latest bibliographic data on file with the International Bureau