A feeding pouch assembly includes a pouch defining a fluid chamber with an outlet port extending from the pouch. The outlet port is in fluid communication with the fluid chamber. A valve is positioned at the outlet port and is biased to a natural sealing position wherein flow from the fluid chamber to the outlet port is prevented. The valve moves to a non-sealing position, wherein fluid is free to flow from the fluid chamber to the outlet, upon connection of the outlet port to a feeding tube. The valve automatically moves to the sealing position upon disconnection of the feeding tube from the outlet port.