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1. (WO2018128836) MODIFIER STREAM ELUTION OF TRAP COLUMN FOR MULTIDIMENSIONAL COMPRESSIBLE FLUID-BASED CHROMATOGRAPHY

Pub. No.:    WO/2018/128836    International Application No.:    PCT/US2017/067820
Publication Date: Fri Jul 13 01:59:59 CEST 2018 International Filing Date: Fri Dec 22 00:59:59 CET 2017
IPC: G01N 30/46
G01N 30/34
G01N 30/20
Applicants: WATERS TECHNOLOGIES CORPORATION
Inventors: FOGWILL, Michael O.
FRANÇOIS, Isabelle
Title: MODIFIER STREAM ELUTION OF TRAP COLUMN FOR MULTIDIMENSIONAL COMPRESSIBLE FLUID-BASED CHROMATOGRAPHY
Abstract:
A method and a device for performing a chromatographic separation include modulating a portion of a flowstream to a trap column (24) to retain at least one analyte from the flowstream on said trap column (24). A flow of a modifier (Mod) is provided through the trap column to generate an elution comprising the at least one analyte. A flow of a compressible fluid-based chromatography (CFC) mobile phase or CFC solvent (C02) is merged (34) with the elution from the trap column to generate a diluted elution. Carbon dioxide may be used as the CFC solvent or as a component of the CFC mobile phase. The diluted elution is provided to a CFC column (14) where at least one analyte is focused at a head of the CFC column. Examples of a flowstream that may be used include an eluent from a chromatography column or a fluid flow from an extraction system.