Some content of this application is unavailable at the moment.
If this situation persist, please contact us atFeedback&Contact
1. (WO2018128586) METHODS OF GENERATING HEPATIC MACROPHAGES AND USES THEREOF
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2018/128586 International Application No.: PCT/SG2018/050007
Publication Date: 12.07.2018 International Filing Date: 05.01.2018
IPC:
C12N 5/0786 (2010.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
078
Cells from blood or from the immune system
0786
Monocytes; Macrophages
Applicants:
AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH [SG/SG]; 1 Fusionopolis Way #20-10, Connexis North Tower, Singapore 138632, SG
Inventors:
TASNIM, Farah; SG
YU, Hanry; SG
Agent:
SPRUSON & FERGUSON (ASIA) PTE LTD; P.O. Box 1531, Robinson Road Post Office Singapore 903031, SG
Priority Data:
10201700068V05.01.2017SG
Title (EN) METHODS OF GENERATING HEPATIC MACROPHAGES AND USES THEREOF
(FR) PROCÉDÉS DE GÉNÉRATION DE MACROPHAGES HÉPATIQUES ET UTILISATIONS CORRESPONDANTES
Abstract:
(EN) The present disclosure provides a method of deriving hepatic macrophages from stem cell-derived monocytes, through the use of hepatic macrophage culture medium comprising a hepatocyte conditioned medium and a basal medium, wherein the conditioned medium is obtained through culturing hepatocytes in a serum-free culture medium in the presence of an extracellular matrix. Also disclosed is a kit used for such a method and hepatic macrophages derived using the method and uses thereof.
(FR) La présente invention concerne un procédé de dérivation de macrophages hépatiques à partir de monocytes dérivés de cellules souches, par l'utilisation d'un milieu de culture de macrophages hépatiques comprenant un milieu conditionné d'hépatocytes et un milieu de base, le milieu conditionné étant obtenu par culture d'hépatocytes dans un milieu de culture exempt de sérum en présence d'une matrice extracellulaire. L'invention concerne également un kit utilisé pour un tel procédé et les macrophages hépatiques dérivés à l'aide du procédé et des utilisations correspondantes.
front page image
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)