An automatic analysis device and a sample analysis method. The automatic analysis device (100) comprises: a loading unit (20) for loading a sample and/or a reagent to a reaction vessel; a reaction unit (10) for incubating, and washing and separating reactants in a reaction vessel; a measurement unit (80) for measuring reaction signals in the reaction vessel; and a transferring unit (50) for transferring the reaction vessel between different locations. The device (100) realizes incubation, washing and separation of the reactants in the reaction vessel by utilizing the reaction unit (10) as a center. The measurement unit (80) independent of the reaction unit (10) measures the signals in the reaction vessel. The present invention not only eliminates the need to separately wash and separate plates, but also simplifies the system structure and control flow. Furthermore, the size of the reaction unit (10) can be significantly reduced and a flexible incubation time can also be realized. In addition, the separate measurement unit (80) makes it easier to achieve a darkroom environment and enables flexible signal incubation times.