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1. (WO2018126499) METHOD FOR PREPARING RETICULOCYTE SIMULATED PARTICLE AND PLATELET SIMULATED PARTICLE, AND QUALITY CONTROL MATERIAL

Pub. No.:    WO/2018/126499    International Application No.:    PCT/CN2017/071755
Publication Date: Fri Jul 13 01:59:59 CEST 2018 International Filing Date: Fri Jan 20 00:59:59 CET 2017
IPC: G01N 33/49
G01N 33/96
Applicants: SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS CO., LTD.
深圳迈瑞生物医疗电子股份有限公司
Inventors: LIU, Bo
刘博
XIE, Jian
谢键
CHEN, Gengwen
陈庚文
SONG, Ruixia
宋瑞霞
Title: METHOD FOR PREPARING RETICULOCYTE SIMULATED PARTICLE AND PLATELET SIMULATED PARTICLE, AND QUALITY CONTROL MATERIAL
Abstract:
Provided are a method for preparing reticulocyte simulated particles and platelet simulated particles, and a quality control material. The method for preparing the reticulocyte simulated particles uses an N-hydroxysuccinimide-activated protein fluorescent dye to stain mammalian enucleated red blood cells with a volume of 60-120 fL and performs the fixed treatment to the enucleated red blood cells to prepare reticulocyte simulated particles. The platelet simulated particles are prepared using mammalian enucleated red blood cells with a volume of 2-25 fL, and the rest is the same as that for the reticulocyte simulated particles. The preparation method comprises: using an N-hydroxysuccinimide-activated protein fluorescent dye to stain different volumes of mammalian enucleated red blood cells, and obtaining reticulocyte simulated particles and platelet simulated particles respectively. The scatter diagram fluorescence and volume direction thereof are close to the scatter diagram distribution of reticulocytes, reticulated platelets, and platelets in fresh blood. The prepared simulated particles have good stability and do not interfere with the count classification of other cells and particles.