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1. (WO2018126469) METHOD FOR PREPARING URSODEOXYCHOLIC ACID AND ENZYME 2 FOR USE IN PREPARATION THEREOF

Pub. No.:    WO/2018/126469    International Application No.:    PCT/CN2017/070605
Publication Date: Fri Jul 13 01:59:59 CEST 2018 International Filing Date: Tue Jan 10 00:59:59 CET 2017
IPC: C12P 33/00
Applicants: BONTAC INSTITUTE OF GREEN BIOCATALYSIS
深圳市邦泰绿色生物合成研究院
Inventors: LIU, Lihui
刘立辉
FU, Rongzhao
傅荣昭
LIU, Taotao
刘滔滔
CAO, Lei
曹磊
PENG, Ting
彭亭
Title: METHOD FOR PREPARING URSODEOXYCHOLIC ACID AND ENZYME 2 FOR USE IN PREPARATION THEREOF
Abstract:
The present invention relates to a method for preparing ursodeoxycholic acid by using a biological enzyme catalysis technique and a 7β-steroid dehydrogenase for use in the preparation thereof. The method uses 3α-hydroxy-7 oxo-5β-cholanic acid as a substrate, and catalyzes 3α-hydroxy-7 oxo-5β-cholanic acid with a 7β-steroid dehydrogenase in the presence of NADP, glucose, a glucose dehydrogenase and a buffer solution to prepare ursodeoxycholic acid, wherein the 7β-steroid dehydrogenase is derived from Enterococcus silesiacus. The method is simple to operate, and has mild and easy-to-control reaction conditions and short reaction time. The substrate conversion efficiency is up to over 99.6%, and the content of the obtained product is above 98.5%.