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1. WO2018126467 - PREPARATION METHOD FOR 3α-HYDROXY-7-OXO-5β-CHOLANOIC ACID AND ENZYME 2 FOR PREPARATION THEREOF

Publication Number WO/2018/126467
Publication Date 12.07.2018
International Application No. PCT/CN2017/070603
International Filing Date 09.01.2017
IPC
C12P 33/02 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
33Preparation of steroids
02Dehydrogenating; Dehydroxylating
C12N 9/04 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02Oxidoreductases (1.), e.g. luciferase
04acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
CPC
C12P 33/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
33Preparation of steroids
02Dehydrogenating; Dehydroxylating
Applicants
  • 深圳市邦泰绿色生物合成研究院 BONTAC INSTITUTE OF GREEN BIOCATALYSIS [CN]/[CN]
Inventors
  • 傅荣昭 FU, Rongzhao
  • 刘立辉 LIU, Lihui
  • 曹磊 CAO, Lei
  • 刘滔滔 LIU, Taotao
  • 彭亭 PENG, Ting
Priority Data
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) PREPARATION METHOD FOR 3α-HYDROXY-7-OXO-5β-CHOLANOIC ACID AND ENZYME 2 FOR PREPARATION THEREOF
(FR) PROCÉDÉ DE PRÉPARATION D'ACIDE 3α-HYDROXY-7-OXO-5β-CHOLANOÏQUE ET ENZYME 2 POUR SA PRÉPARATION
(ZH) 一种3α-羟基-7氧代-5β-胆烷酸的制备方法及其制备用酶2
Abstract
(EN)
A method which uses biological enzyme catalysis to prepare 3α-hydroxy-7-oxo-5β-cholanoic acid and 7α-steroid dehydrogenase for the preparation thereof. The method uses chenodeoxycholic acid as a substrate; in the presence of NAD, lactate dehydrogenase, sodium pyruvate and a buffer solution, the 7α-steroid dehydrogenase is used to catalyze the chenodeoxycholic acid to prepare the 3α-hydroxy-7-oxo-5β-cholanoic acid, wherein the 7α-steroid dehydrogenase is derived from Cyanothece sp. ATCC 29155.
(FR)
L'invention un procédé qui utilise une catalyse enzymatique biologique pour préparer l'acide 3α-hydroxy-7-oxo-5β-cholanoïque et la 7α-stéroïde déshydrogénase pour sa préparation. Le procédé utilise de l'acide chénodésoxycholique en tant que substrat; en présence de NAD, de lactate déshydrogénase, de pyruvate de sodium et d'une solution tampon, la 7α-stéroïde déshydrogénase est utilisée pour catalyser l'acide chénodésoxycholique pour préparer l'acide 3α-hydroxy-7-oxo-5β-cholanoïque, la 7α-stéroïde déshydrogénase étant dérivée de cyanothécène sp. ATCC 29155.
(ZH)
一种利用生物酶催化制备3α-羟基-7氧代-5β-胆烷酸的方法及其制备用7α-类固醇脱氢酶。该方法以鹅去氧胆酸为底物,在NAD、乳酸脱氢酶、丙酮酸钠以及缓冲溶液存在的条件下,用7α-类固醇脱氢酶催化鹅去氧胆酸制备3α-羟基-7氧代-5β胆烷酸,其中7α-类固醇脱氢酶来源于蓝杆藻Cyanothece sp.ATCC 29155。
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