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1. (WO2018126287) CELL-FREE EXPRESSION SYSTEM HAVING NOVEL INORGANIC POLYPHOSPHATE-BASED ENERGY REGENERATION

Pub. No.:    WO/2018/126287    International Application No.:    PCT/US2018/012121
Publication Date: Fri Jul 06 01:59:59 CEST 2018 International Filing Date: Wed Jan 03 00:59:59 CET 2018
IPC: C12N 15/09
C12N 5/10
C12N 1/20
C12N 9/22
C12N 15/113
C12Q 1/68
Applicants: NTXBIO, LLC
Inventors: KOGLIN, Alexander
HUMBERT, Michael
Title: CELL-FREE EXPRESSION SYSTEM HAVING NOVEL INORGANIC POLYPHOSPHATE-BASED ENERGY REGENERATION
Abstract:
The invention relates to an in vitro cell-free expression system incorporating a novel inorganic polyphosphate-based energy regeneration system. In certain embodiments, the invention includes a cell-free expression system where the cellular energy source, ATP, is regenerated from inorganic polyphosphate using a dual enzyme system. In this embodiment, this dual enzyme system may include thermostable Adenosyl Kinase, and/or Polyphosphate Kinase enzymes.