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1. (WO2018126266) PHAGE-MEDIATED IMMUNOASSAY AND METHODS FOR DETERMINING SUSCEPTIBILITY OF BACTERIA TO ANTIBIOTIC OR PROBIOTIC AGENTS

Pub. No.:    WO/2018/126266    International Application No.:    PCT/US2018/012071
Publication Date: Fri Jul 06 01:59:59 CEST 2018 International Filing Date: Wed Jan 03 00:59:59 CET 2018
IPC: C12Q 1/04
G01N 33/569
Applicants: QUIDEL COROPORATION
Inventors: BELENKY, Alexander
Title: PHAGE-MEDIATED IMMUNOASSAY AND METHODS FOR DETERMINING SUSCEPTIBILITY OF BACTERIA TO ANTIBIOTIC OR PROBIOTIC AGENTS
Abstract:
Methods for determining the susceptibility or resistance of bacteria to antibiotic agents are provided. In one embodiment, the methods include culturing the bacteria in the presence or absence or the antimicrobial agent to generate a primary culture which is then cultured in the presence or absence of transforming phages. The recombinant phages are specific to the bacteria and comprise a heterologous marker (e.g., a nucleic acid that is expressible as a detectable product such as an RNA or a protein). The susceptibility or resistance of the bacteria to the antimicrobial agent may be determined by assaying the culture for the presence or absence of the heterologous marker, wherein a reduction in the level or activity of the marker in the culture compared to the level or activity of the marker in a comparative culture indicates that the bacteria is sensitive to the antibiotic agent.