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1. (WO2018126207) A METHOD TO BUILD FUNGAL PRODUCTION STRAINS USING AUTOMATED STEPS FOR GENETIC MANIPULATION AND STRAIN PURIFICATION

Pub. No.:    WO/2018/126207    International Application No.:    PCT/US2017/069086
Publication Date: Fri Jul 06 01:59:59 CEST 2018 International Filing Date: Sat Dec 30 00:59:59 CET 2017
IPC: C12N 15/80
C12N 15/09
C12N 15/90
Applicants: ZYMERGEN INC.
Inventors: BRUNO, Kenneth S.
WESTFALL, Patrick
SZEWCZYK, Edyta
ROTHSCHILD-MANCINELLI, Kyle
Title: A METHOD TO BUILD FUNGAL PRODUCTION STRAINS USING AUTOMATED STEPS FOR GENETIC MANIPULATION AND STRAIN PURIFICATION
Abstract:
The present disclosure provides a high-throughput (HTP) microbial genomic engineering method and system for transforming, screening, and selecting filamentous fungal cells that utilizes automation. The method and system utilize HTP selection and counter-selection to purify homokaryotic transformed filamentous fungal cells. Furthermore, the present disclosure provides a method for producing and long-term storage of protoplasts derived from filamentous fungal cells.