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1. (WO2018125865) COMBINED LYSIS PROTOCOL FOR COMPREHENSIVE CELL LYSIS
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Pub. No.: WO/2018/125865 International Application No.: PCT/US2017/068415
Publication Date: 05.07.2018 International Filing Date: 26.12.2017
IPC:
B01F 13/02 (2006.01) ,C12M 1/04 (2006.01) ,C12N 1/06 (2006.01)
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
F
MIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
13
Other mixers; Mixing plant, including combinations of dissimilar mixers
02
Mixers with gas agitation, e.g. with air supply tubes
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
04
with gas introduction means
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
06
Lysis of micro-organisms
Applicants:
SHORELINE BIOME, LLC [US/US]; 400 Farmington Avenue CB 119 Farmington, CO 06032, US
Inventors:
DRISCOLL, Mark; US
JARVIE, Thomas; US
Agent:
BOCHNER, Andrew, D.; US
Priority Data:
62/440,17129.12.2016US
Title (EN) COMBINED LYSIS PROTOCOL FOR COMPREHENSIVE CELL LYSIS
(FR) PROTOCOLE DE LYSE COMBINÉ POUR LYSE CELLULAIRE COMPLÈTE
Abstract:
(EN) Disclosed are methods for lysis of cells, such as bacteria present in microbiomes, that combine three lysis steps— (1) heat, (2) detergent and (3) base— into a single step and that can be completed in a short period of time, e.g., a few minutes. The methods combine a normally incompatible detergent and base lysis, allows for simplified removal of detergent after lysis, and importantly, yields improved quantities of genomic DNA (gDNA) from difficult to lyse bacteria.
(FR) L'invention concerne des procédés de lyses cellulaires, telles que des bactéries présentes dans des microbiomes. Ces procédés combinent trois étapes de lyse -(1) chaleur, (2) détergent et (3) base - en une seule étape et peuvent être achevés en une courte période de temps, par exemple quelques minutes. Les procédés combinent un détergent normalement incompatible et une lyse de base, permettent un retrait simplifié du détergent après lyse, et de manière importante, permettent d'obtenir des quantités améliorées d'ADN génomique (gDNA) à partir de bactéries difficiles à lyser.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)