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1. (WO2018125863) FLUORESCENCE-BASED REPORTERS FOR MUTAGENESIS DETECTION IN E. COLI

Pub. No.:    WO/2018/125863    International Application No.:    PCT/US2017/068410
Publication Date: Fri Jul 06 01:59:59 CEST 2018 International Filing Date: Wed Dec 27 00:59:59 CET 2017
IPC: C12N 15/63
C12Q 1/10
C12Q 1/37
G01N 21/64
G01N 33/573
Applicants: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventors: CAMPS, Manel
ALLEN, Jennifer
Title: FLUORESCENCE-BASED REPORTERS FOR MUTAGENESIS DETECTION IN E. COLI
Abstract:
Direct detection of mutagenesis in prokaryotes by reversion of an inactivating mutation (reversion mutation assay), producing a quantitative signal for in vivo mutagenesis, may greatly reduce the amount of test chemicals and labor involved in these assays. Further, transcriptional coupling of β-lactamase reversion and GFP, translational fusion between β-lactamase and GFP with stop codon in GFP, and a novel dual reporter to monitor continuous mutagenesis may be used in methods described herein.