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|1. (WO2018125759) METHOD AND SYSTEM EMPLOYING DISTINGUISHABLE POLYMERASES FOR DETECTING TERNARY COMPLEXES AND IDENTIFYING COGNATE NUCLEOTIDES|
|Inventors:||DAMBACHER, Corey M.
|Title:||METHOD AND SYSTEM EMPLOYING DISTINGUISHABLE POLYMERASES FOR DETECTING TERNARY COMPLEXES AND IDENTIFYING COGNATE NUCLEOTIDES|
Method of identifying a cognate nucleotide (i.e., the "next correct nucleotide") for a primed template nucleic acid molecule. In some embodiments, an ordered or random array of primed target nucleic acids characterized by different cognate nucleotides can be evaluated using a single imaging step to identify different cognate nucleotides for a collection of different primed template nucleic acid molecules. An optional incorporation step can follow the identifying step. A polymerase different from the ones used in the binding and examination steps can be used to incorporate a nucleotide, such as a reversible terminator nucleotide, preliminary to identification of the next cognate nucleotide.