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1. (WO2018125759) METHOD AND SYSTEM EMPLOYING DISTINGUISHABLE POLYMERASES FOR DETECTING TERNARY COMPLEXES AND IDENTIFYING COGNATE NUCLEOTIDES

Pub. No.:    WO/2018/125759    International Application No.:    PCT/US2017/067976
Publication Date: Fri Jul 06 01:59:59 CEST 2018 International Filing Date: Fri Dec 22 00:59:59 CET 2017
IPC: C12Q 1/6869
C12Q 1/6827
C12Q 1/6874
Applicants: OMNIOME, INC.
Inventors: DAMBACHER, Corey M.
CAYER, Devon
LECOULTRE, Richard
ROKICKI, Joseph
WILSON, Kerry
TU, Eugene
VIJAYAN, Kandaswamy
Title: METHOD AND SYSTEM EMPLOYING DISTINGUISHABLE POLYMERASES FOR DETECTING TERNARY COMPLEXES AND IDENTIFYING COGNATE NUCLEOTIDES
Abstract:
Method of identifying a cognate nucleotide (i.e., the "next correct nucleotide") for a primed template nucleic acid molecule. In some embodiments, an ordered or random array of primed target nucleic acids characterized by different cognate nucleotides can be evaluated using a single imaging step to identify different cognate nucleotides for a collection of different primed template nucleic acid molecules. An optional incorporation step can follow the identifying step. A polymerase different from the ones used in the binding and examination steps can be used to incorporate a nucleotide, such as a reversible terminator nucleotide, preliminary to identification of the next cognate nucleotide.