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1. (WO2018124235) METHOD FOR DETECTING MITOCHONDRIAL tRNA MODIFICATION

Pub. No.:    WO/2018/124235    International Application No.:    PCT/JP2017/047099
Publication Date: Fri Jul 06 01:59:59 CEST 2018 International Filing Date: Thu Dec 28 00:59:59 CET 2017
IPC: G01N 27/62
C12Q 1/68
G01N 33/50
Applicants: NATIONAL UNIVERSITY CORPORATION KUMAMOTO UNIVERSITY
国立大学法人熊本大学
Inventors: TOMIZAWA, Kazuhito
富沢 一仁
WEI, Fanyan
魏 范研
Title: METHOD FOR DETECTING MITOCHONDRIAL tRNA MODIFICATION
Abstract:
The purpose of the present invention is to provide a method for detecting a modified nucleoside of mitochondrial transition RNA (mt-tRNA). The present invention provides a method for detecting a modified nucleoside in mt-tRNA, the method using tandem mass analysis, and including detecting a modified nucleoside (for example, 5-taurinomethyl-2-thiouridine (τ m5s2U)), 5-taurinomethyluridine (τ m5U), or 2-methylthio-N6-isopentenyl adenosine (ms2i6A)) in a specimen of a bodily fluid such as urine or in a specimen in a culture supernatant.