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1. WO2018124210 - RING-SHAPED CARDIAC MUSCLE CELL MASS

Publication Number WO/2018/124210
Publication Date 05.07.2018
International Application No. PCT/JP2017/047027
International Filing Date 27.12.2017
IPC
C12N 5/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
A61P 9/00 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
9Drugs for disorders of the cardiovascular system
C12M 3/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
C12N 5/077 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
077Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
C12N 5/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C12Q 1/02 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02involving viable microorganisms
CPC
A61P 9/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
9Drugs for disorders of the cardiovascular system
C12M 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
3Tissue, human, animal or plant cell, or virus culture apparatus
C12N 5/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
C12N 5/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
10Cells modified by introduction of foreign genetic material
C12Q 1/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
02involving viable microorganisms
Applicants
  • 国立大学法人京都大学 KYOTO UNIVERSITY [JP]/[JP]
Inventors
  • 劉 莉 LIU, Li
  • 李 俊君 LI, Junjun
Agents
  • 鮫島 睦 SAMEJIMA, Mutsumi
  • 櫻井 陽子 SAKURAI, Yoko
Priority Data
2016-25525828.12.2016JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) RING-SHAPED CARDIAC MUSCLE CELL MASS
(FR) MASSE ANNULAIRE DE CELLULES DE MUSCLE CARDIAQUE
(JA) 環状心筋細胞塊
Abstract
(EN)
The present disclosure provides a ring-shaped cardiac muscle cell mass in which an electric signal or a calcium signal can progress while circulating therethrough. Also provided is a method for producing the ring-shaped cardiac muscle cell mass, said method comprising a step of culturing cardiac muscle cells induced from pluripotent stem cells in a culture vessel having a protruded part formed at the inside bottom thereof. Also provided is a culture vessel having a protruded part formed at the inside bottom thereof. Also provided are: a method for assessing the cardiotoxicity of a candidate substance for a medicine and a method for screening for a therapeutic agent for a heart disease, in each of which the ring-shaped cardiac muscle cell mass is used; and a kit for the method, which includes the ring-shaped cardiac muscle cell mass.
(FR)
La présente invention concerne une masse annulaire de cellules de muscle cardiaque dans laquelle un signal électrique ou un signal calcique peut progresser lorsqu'il y circule. L'invention concerne également une méthode permettant de produire la masse annulaire de cellules de muscle cardiaque, ladite méthode comprenant une étape de culture de cellules de muscle cardiaque induites à partir de cellules souches pluripotentes dans un récipient de culture présentant une partie en saillie formée au niveau de son fond intérieur. L'invention concerne également un récipient de culture présentant une partie en saillie formée au niveau de son fond intérieur. L'invention concerne également : une méthode d'évaluation de la cardiotoxicité de substances candidates pour une utilisation comme médicament ainsi qu'une méthode de criblage d'agents thérapeutiques contre des maladies cardiaques, chacune de ces méthodes utilisant la masse annulaire de cellules de muscle cardiaque ; et un kit pour la méthode, comprenant la masse annulaire de cellules de muscle cardiaque.
(JA)
本開示は、電気信号またはカルシウム信号が環状心筋細胞塊内を循環して進行している環状心筋細胞塊を提供する。また、多能性幹細胞から誘導された心筋細胞を、内側底部に凸部を有する培養容器中で培養する工程を含む、当該環状心筋細胞塊の製造方法を提供する。さらに、内側底部に凸部を有する培養容器を提供する。また、当該環状心筋細胞塊を使用して、医薬候補物質の心毒性を評価する方法および心疾患の処置剤をスクリーニングする方法、並びに、当該環状心筋細胞塊を含むそのためのキットを提供する。
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