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1. (WO2018124189) METHOD OF SELECTING CELLS
Latest bibliographic data on file with the International BureauSubmit observation

Pub. No.: WO/2018/124189 International Application No.: PCT/JP2017/046939
Publication Date: 05.07.2018 International Filing Date: 27.12.2017
IPC:
C12N 15/09 (2006.01) ,C12N 1/15 (2006.01) ,C12N 1/19 (2006.01) ,C12N 1/21 (2006.01) ,C12N 5/10 (2006.01) ,C12P 21/00 (2006.01) ,C12P 21/08 (2006.01) ,C12Q 1/04 (2006.01) ,C12Q 1/68 (2018.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
14
Fungi; Culture media therefor
15
modified by introduction of foreign genetic material
C CHEMISTRY; METALLURGY
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
14
Fungi; Culture media therefor
16
Yeasts; Culture media therefor
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modified by introduction of foreign genetic material
C CHEMISTRY; METALLURGY
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
20
Bacteria; Culture media therefor
21
modified by introduction of foreign genetic material
C CHEMISTRY; METALLURGY
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10
Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C CHEMISTRY; METALLURGY
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21
Preparation of peptides or proteins
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21
Preparation of peptides or proteins
08
Monoclonal antibodies
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
04
Determining presence or kind of micro-organism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
協和発酵キリン株式会社 KYOWA HAKKO KIRIN CO., LTD. [JP/JP]; 東京都千代田区大手町一丁目6番1号 1-6-1, Otemachi, Chiyoda-ku, Tokyo 1008185, JP
Inventors:
山本 耕一 YAMAMOTO Koichi; JP
松本 悠一 MATSUMOTO Yuichi; JP
山口 恵奈 YAMAGUCHI Keina; JP
藤井 滉人 FUJII Hiroto; JP
須澤 敏行 SUZAWA Toshiyuki; JP
Agent:
特許業務法人栄光特許事務所 Eikoh Patent Firm, P.C.; JP
Priority Data:
2016-25627928.12.2016JP
Title (EN) METHOD OF SELECTING CELLS
(FR) MÉTHODE DE SÉLECTION DE CELLULES
(JA) 細胞を選抜する方法
Abstract:
(EN) The purpose of the present invention is to provide a method of selecting cell lines in which the reduction of a recombinant protein has been suppressed. The present invention pertains to a method of selecting cells, said method comprising a first step in which the intracellular expression level of at least one gene selected from among genes having any one of the base sequences represented by SEQ ID NO: 1-16 and orthologs thereof is measured, and a second step in which the expression level achieved by suppressing the reduction of a recombinant protein is evaluated on the basis of the difference determined by comparing the gene expression level measured in the first step and a control value for the gene expression level.
(FR) L'objectif de la présente invention est de fournir une méthode permettant de sélectionner des lignées cellulaires dans lesquelles la diminution d'une protéine recombinante a été empêchée. La présente invention concerne une méthode permettant de sélectionner des cellules, ladite méthode comprenant une première étape lors de laquelle est mesuré le niveau d'expression intracellulaire d'au moins un gène sélectionné parmi les gènes ayant l'une quelconque des séquences de base représentées par les séquences SEQ ID NO : 1-16 et des orthologues de ces derniers, et une seconde étape lors de laquelle le niveau d'expression obtenu en empêchant la diminution d'une protéine recombinante est évalué sur la base de la différence déterminée en comparant le niveau d'expression du gène mesuré à la première étape et une valeur de contrôle du niveau d'expression du gène.
(JA) 本発明は、組換えタンパク質の還元が抑制された細胞株を選抜する方法を提供することを目的とする。本発明は、第1工程:配列番号1~16に示される塩基配列のいずれか1を有する遺伝子又はそのオーソログな遺伝子から選ばれる少なくとも1の遺伝子の細胞内の当該遺伝子の発現レベルを測定する工程、および第2工程:前記第1工程において測定した前記遺伝子の発現レベルを、前記遺伝子の発現レベルの対照値と比較し、その差異に基づき、組換えタンパク質の還元を抑制し得る発現レベルを評価する工程を含む、細胞を選抜する方法に関する。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)