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1. (WO2018123966) METHOD FOR HARVESTING CULTURED CELLS FROM THREE-DIMENSIONAL POROUS SCAFFOLD

Pub. No.:    WO/2018/123966    International Application No.:    PCT/JP2017/046420
Publication Date: Fri Jul 06 01:59:59 CEST 2018 International Filing Date: Tue Dec 26 00:59:59 CET 2017
IPC: C12M 3/04
C12N 5/00
Applicants: FULLSTEM CO., LTD.
株式会社フルステム
Inventors: CHIBA Shunmei
千葉 俊明
Title: METHOD FOR HARVESTING CULTURED CELLS FROM THREE-DIMENSIONAL POROUS SCAFFOLD
Abstract:
Provided is a method for efficiently harvesting cells cultured at a large scale from a three-dimensional porous scaffold. This method includes: a culturing step in which cells are cultured on a three-dimensional porous scaffold sealed in a culture vessel; an enzymatic treatment step in which an enzyme solution containing trypsin-EDTA, caseinase, and/or collagenase is added to the culture vessel to conduct an enzymatic treatment on the cultured cells in the culture vessel; and a vibration treatment step in which a vibration treatment is conducted, i.e., a base that makes a circular motion within a horizontal plane and a bottom surface of the culture vessel are made to come into surface contact with one another so as to impart a vertical-direction vibration to the cultured cells that have undergone the enzymatic treatment.