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1. (WO2018122777) PEPTIDE SEQUENCE OF A GUIDE PROTEIN FOR THE PRODUCTION OF A PEPTIDE OF INTEREST, AN EXPRESSION VECTOR, A HOST CELL, AND A METHOD FOR THE PRODUCTION OF A PEPTIDE OF INTEREST

Pub. No.:    WO/2018/122777    International Application No.:    PCT/IB2017/058480
Publication Date: Fri Jul 06 01:59:59 CEST 2018 International Filing Date: Fri Dec 29 00:59:59 CET 2017
IPC: C07K 14/46
C12P 21/02
C12N 15/62
C12N 15/67
Applicants: UNIVERSIDAD DE CHILE
Inventors: ASENJO DE LEUZE, Juan Adolfo
ANDREWS FARROW, Bárbara Anne
RODRÍGEZ GALLARDO, Vida
Title: PEPTIDE SEQUENCE OF A GUIDE PROTEIN FOR THE PRODUCTION OF A PEPTIDE OF INTEREST, AN EXPRESSION VECTOR, A HOST CELL, AND A METHOD FOR THE PRODUCTION OF A PEPTIDE OF INTEREST
Abstract:
The invention relates to a peptide sequence of a guide protein for the production of a peptide of interest; a peptide sequence with a degree of similarity of at least 90% with the former; a nucleotide sequence which codifies for said guide protein; an expression vector comprising said nucleotide sequence; a host cell that expresses a fusion protein comprising a peptide of interest; and a method for producing a peptide of interest comprising the following steps: A) constructing an expression vector; B) inserting the expression vector into a host cell; C) expressing the fusion protein, cultivating the host cell in a culture medium; D) recovering the fusion protein accumulated in the host cell; E) separating the fusion protein; and F) purifying the peptide of interest.