Some content of this application is unavailable at the moment.
If this situation persist, please contact us atFeedback&Contact
1. (WO2018122401) TETHERED PARTICLE BIOSENSOR WITH DIFFERENT CAPTURE MOLECULES FOR THE DETECTION OF SWITCHING EVENTS
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2018/122401 International Application No.: PCT/EP2018/050019
Publication Date: 05.07.2018 International Filing Date: 02.01.2018
IPC:
G01N 33/543 (2006.01) ,G01N 33/542 (2006.01) ,G01N 33/557 (2006.01) ,C12Q 1/6825 (2018.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
543
with an insoluble carrier for immobilising immunochemicals
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
536
with immune complex formed in liquid phase
542
with steric inhibition or signal modification, e.g. fluorescent quenching
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
557
using kinetic measurement, i.e. time rate of progress of an antigen-antibody interaction
[IPC code unknown for C12Q 1/6825]
Applicants:
TECHNISCHE UNIVERSITEIT EINDHOVEN [NL/NL]; Den Dolech 2 5612 AZ Eindhoven, NL
Inventors:
VISSER, Emillius Willem Adriaan; NL
VAN IJZENDOORN, Leonardus Josephus; NL
PRINS, Menno Willem José; NL
Agent:
FRKELLY; 27 Clyde Road Dublin, D04 F838, IE
Priority Data:
62/439,83328.12.2016US
Title (EN) TETHERED PARTICLE BIOSENSOR WITH DIFFERENT CAPTURE MOLECULES FOR THE DETECTION OF SWITCHING EVENTS
(FR) BIOCAPTEUR À PARTICULES CAPTIVES COMPORTANT DIFFÉRENTES MOLÉCULES DE CAPTURE POUR LA DÉTECTION D'ÉVÉNEMENTS DE COMMUTATION
Abstract:
(EN) A method for biosensing a target substance [110] uses a collection of particles [104] tethered to a surface [100] by tether molecules [102]. First capture molecules [106] are attached to the particles [104] or to the tether molecules. Second capture molecules [108] are attached to the surface [100] or to the tether molecules. The first capture molecules [106] are selected to bind to the target substance [110] and have a first target dissociation rate, and the second capture molecules [108] are selected to bind to the target substance [110] and have a second target dissociation rate that differs from the first target dissociation rate by at least a factor of three. A time series of a spatial coordinate parameter of the particles is detected, and identifying a time sequence of individual association/dissociation events of the target substance with the capture molecules are identified within the detected time series. Each of the events corresponds to a change between binding states of the first capture molecule [106] and the second capture molecule [108] to the target substance [110]. A concentration of the target substance is determined from the identified time sequence of individual association/dissociation events. Competitive assay configurations are also described.
(FR) La présente invention concerne un procédé de biodétection d'une substance cible [110] faisant appel à une collection de particules [104] fixées à une surface [100] par des molécules d'attache [102]. Des premières molécules de capture [106] sont fixées aux particules [104] ou aux molécules d'attache. Des secondes molécules de capture [108] sont fixées à la surface [100] ou aux molécules d'attache. Les premières molécules de capture [106] sont sélectionnées pour se lier à la substance cible [110] et présentent une première vitesse de dissociation cible, et les secondes molécules de capture [108] sont sélectionnées pour se lier à la substance cible [110] et présente une seconde vitesse de dissociation cible qui diffère de la première vitesse de dissociation cible d'au moins un facteur trois. Une série chronologique d'un paramètre de coordonnées spatiales des particules est détectée, et une séquence temporelle d'événements d'association/dissociation individuels de la substance cible avec les molécules de capture est identifiée dans la série chronologique détectée. Chacun des événements correspond à un changement entre des états de liaison de la première molécule de capture [106] et de la seconde molécule de capture [108] à la substance cible [110]. Une concentration de la substance cible est déterminée à partir de la séquence chronologique identifiée d'événements d'association/dissociation individuels. L'invention concerne également des configurations de dosage compétitif.
front page image
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)