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1. WO2018122401 - TETHERED PARTICLE BIOSENSOR WITH DIFFERENT CAPTURE MOLECULES FOR THE DETECTION OF SWITCHING EVENTS

Publication Number WO/2018/122401
Publication Date 05.07.2018
International Application No. PCT/EP2018/050019
International Filing Date 02.01.2018
IPC
G01N 33/543 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
G01N 33/542 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
536with immune complex formed in liquid phase
542with steric inhibition or signal modification, e.g. fluorescent quenching
G01N 33/557 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
557using kinetic measurement, i.e. time rate of progress of an antigen-antibody interaction
C12Q 1/6825 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6813Hybridisation assays
6816characterised by the detection means
6825Nucleic acid detection involving sensors
CPC
C12Q 1/6834
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
G01N 21/63
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
G01N 33/542
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
536with immune complex formed in liquid phase
542with steric inhibition or signal modification, e.g. fluorescent quenching
G01N 33/543
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
G01N 33/557
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
557using kinetic measurement, i.e. time rate of progress of an antigen-antibody interaction
Applicants
  • TECHNISCHE UNIVERSITEIT EINDHOVEN [NL]/[NL]
Inventors
  • VISSER, Emillius Willem Adriaan
  • VAN IJZENDOORN, Leonardus Josephus
  • PRINS, Menno Willem José
Agents
  • FRKELLY
Priority Data
62/439,83328.12.2016US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) TETHERED PARTICLE BIOSENSOR WITH DIFFERENT CAPTURE MOLECULES FOR THE DETECTION OF SWITCHING EVENTS
(FR) BIOCAPTEUR À PARTICULES CAPTIVES COMPORTANT DIFFÉRENTES MOLÉCULES DE CAPTURE POUR LA DÉTECTION D'ÉVÉNEMENTS DE COMMUTATION
Abstract
(EN) A method for biosensing a target substance [110] uses a collection of particles [104] tethered to a surface [100] by tether molecules [102]. First capture molecules [106] are attached to the particles [104] or to the tether molecules. Second capture molecules [108] are attached to the surface [100] or to the tether molecules. The first capture molecules [106] are selected to bind to the target substance [110] and have a first target dissociation rate, and the second capture molecules [108] are selected to bind to the target substance [110] and have a second target dissociation rate that differs from the first target dissociation rate by at least a factor of three. A time series of a spatial coordinate parameter of the particles is detected, and identifying a time sequence of individual association/dissociation events of the target substance with the capture molecules are identified within the detected time series. Each of the events corresponds to a change between binding states of the first capture molecule [106] and the second capture molecule [108] to the target substance [110]. A concentration of the target substance is determined from the identified time sequence of individual association/dissociation events. Competitive assay configurations are also described.
(FR) La présente invention concerne un procédé de biodétection d'une substance cible [110] faisant appel à une collection de particules [104] fixées à une surface [100] par des molécules d'attache [102]. Des premières molécules de capture [106] sont fixées aux particules [104] ou aux molécules d'attache. Des secondes molécules de capture [108] sont fixées à la surface [100] ou aux molécules d'attache. Les premières molécules de capture [106] sont sélectionnées pour se lier à la substance cible [110] et présentent une première vitesse de dissociation cible, et les secondes molécules de capture [108] sont sélectionnées pour se lier à la substance cible [110] et présente une seconde vitesse de dissociation cible qui diffère de la première vitesse de dissociation cible d'au moins un facteur trois. Une série chronologique d'un paramètre de coordonnées spatiales des particules est détectée, et une séquence temporelle d'événements d'association/dissociation individuels de la substance cible avec les molécules de capture est identifiée dans la série chronologique détectée. Chacun des événements correspond à un changement entre des états de liaison de la première molécule de capture [106] et de la seconde molécule de capture [108] à la substance cible [110]. Une concentration de la substance cible est déterminée à partir de la séquence chronologique identifiée d'événements d'association/dissociation individuels. L'invention concerne également des configurations de dosage compétitif.
Latest bibliographic data on file with the International Bureau