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1. (WO2018122225) IN VIVO SYNTHESIS OF SIALYLATED COMPOUNDS

Pub. No.:    WO/2018/122225    International Application No.:    PCT/EP2017/084593
Publication Date: Fri Jul 06 01:59:59 CEST 2018 International Filing Date: Wed Dec 27 00:59:59 CET 2017
IPC: C12P 19/26
C12P 19/18
C12N 15/52
C12N 9/16
C12N 9/90
C12N 9/10
C12N 9/80
C12N 9/12
C12N 9/88
C12N 1/21
C12N 1/19
Applicants: INBIOSE N.V.
BEAUPREZ, Joeri
COUSSEMENT, Pieter
VAN HERPE, Dries
PETERS, Gert
VERCAUTEREN, Annelies
Inventors: BEAUPREZ, Joeri
COUSSEMENT, Pieter
VAN HERPE, Dries
PETERS, Gert
VERCAUTEREN, Annelies
Title: IN VIVO SYNTHESIS OF SIALYLATED COMPOUNDS
Abstract:
The present invention is in the technical field of synthetic biology and metabolic engineering. More particularly, the present invention is in the technical field of fermentation of metabolically engineered microorganisms. The present invention describes engineered microorganisms able to synthesize sialylated compounds via an intracellular biosynthesis route. These microorganisms can dephosphorylate N-acetylglucosamine-6-phosphate to N- acetylglucosamine and convert the N-acetylglucosamine to N- acetylmannosamine. These microorganisms also have the ability to convert N-acetylmannosamine to N-acetyl-neuraminate. Furthermore, the present invention provides a method for the large scale in vivo synthesis of sialylated compounds, by culturing a microorganism in a culture medium, optionally comprising an exogenous precursor such as, but not limited to lactose, lactoNbiose, N-acetyllactosamine and/or an aglycon, wherein said microorganism intracellularly dephosphorylates N-acetylglucosamine-6-phosphate to N-acetylglucosamine, converts N-acetylglucosamine to N- acetylmannosamine and convert the latter further to N-acetyl- neuraminate.