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1. (WO2018119848) PCR FLUORESCENCE DETECTOR

Pub. No.:    WO/2018/119848    International Application No.:    PCT/CN2016/112961
Publication Date: Fri Jul 06 01:59:59 CEST 2018 International Filing Date: Fri Dec 30 00:59:59 CET 2016
IPC: C12M 1/38
G01N 33/52
Applicants: SANSURE BIOTECH INC.
湖南圣湘生物科技有限公司
Inventors: DAI, Lizhong
戴立忠
ZHANG, Hongjian
章洪建
DENG, Zhongping
邓中平
TANG, Jingnian
唐景年
YANG, Yong
杨勇
Title: PCR FLUORESCENCE DETECTOR
Abstract:
Provided is a PCR fluorescence detector in which a PCR reaction tube (33) is placed in a first through hole (4), a second through hole (6) and a third through hole (8) so that a bottom of the PCR reaction tube (33) is located at the third through hole (8), and a liquid level of PCR reaction solution in the PCR reaction tube (33) is located at the first through hole (4). Thus a 95°C heating plate (7) heats the bottom of the PCR reaction tube (33), and a 60°C heating plate (5) heats the liquid level of the PCR reaction solution in the PCR reaction tube (33). Thereby, a temperature difference occurs between the upper and lower ends of the PCR reaction tube (33). The temperature difference causes the PCR reaction solution in the PCR reaction tube (33) to flow from a bottom high temperature zone to a top low temperature zone, forming a convection. Because of the temperature difference and the convection, DNA can be continuously amplified in the PCR fluorescence detector, and there is no need to continuously increase the temperature and cool down the temperature to reach the temperature required at different stages of DNA amplification. Therefore, the PCR fluorescence detector can save the amplification time, thereby shortening the detection time of the PCR detector.