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1. WO2018112638 - METHODS AND ORGANISM EXPRESSING METSCHNIKOWIA GENES FOR INCREASED ETHANOL PRODUCTION

Publication Number WO/2018/112638
Publication Date 28.06.2018
International Application No. PCT/CA2017/051561
International Filing Date 20.12.2017
IPC
C12N 1/19 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
14Fungi ; Culture media therefor
16Yeasts; Culture media therefor
19modified by introduction of foreign genetic material
C07C 31/22 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
CACYCLIC OR CARBOCYCLIC COMPOUNDS
31Saturated compounds having hydroxy or O-metal groups bound to acyclic carbon atoms
18Polyhydroxylic acyclic alcohols
22Trihydroxylic alcohols, e.g. glycerol
C07C 47/06 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
CACYCLIC OR CARBOCYCLIC COMPOUNDS
47Compounds having -CHO groups
02Saturated compounds having -CHO groups bound to acyclic carbon atoms or to hydrogen
06Acetaldehyde
C07C 47/19 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
CACYCLIC OR CARBOCYCLIC COMPOUNDS
47Compounds having -CHO groups
02Saturated compounds having -CHO groups bound to acyclic carbon atoms or to hydrogen
19containing hydroxy groups
C07C 53/08 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
CACYCLIC OR CARBOCYCLIC COMPOUNDS
53Saturated compounds having only one carboxyl group bound to an acyclic carbon atom or hydrogen
08Acetic acid
C07K 14/39 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
37from fungi
39from yeasts
CPC
C12N 9/0006
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0006acting on CH-OH groups as donors (1.1)
C12N 9/1022
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
1022transferring aldehyde or ketonic groups (2.2)
C12N 9/1205
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
1205Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
C12N 9/16
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
16acting on ester bonds (3.1)
C12P 7/06
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
02containing a hydroxy group
04acyclic
06Ethanol, i.e. non-beverage
C12Y 101/01175
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
101Oxidoreductases acting on the CH-OH group of donors (1.1)
01with NAD+ or NADP+ as acceptor (1.1.1)
01175D-Xylose 1-dehydrogenase (1.1.1.175)
Applicants
  • CREATUS BIOSCIENCES INC. [CA]/[CA]
Inventors
  • LUO, Zongli
  • JANSEN VAN VUUREN, Hendrick Jurgens
  • DEBONO, Allan George
  • FERGUSON, Andrew Taplin
Agents
  • SMART & BIGGAR
Priority Data
62/437,59621.12.2016US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) METHODS AND ORGANISM EXPRESSING METSCHNIKOWIA GENES FOR INCREASED ETHANOL PRODUCTION
(FR) PROCÉDÉS ET ORGANISME EXPRIMANT DES GÈNES DE METSCHNIKOWIA POUR UNE PRODUCTION ACCRUE D'ÉTHANOL
Abstract
(EN) Provided is a recombinant Saccharomyces cerevisiae strain comprising a xylose-ethanol pathway and expressing at least one exogenous nucleic acid encoding a xylose transporter, a xylose reductase, a xylose dehydrogenase, and a xylulokinase from a Metschnikowia strain deposited at the International Depositary Authority of Canada (IDAC) with Accession Number 081116-01, and additionally comprising a transaldolase and deletion of PH013, and method for production of ethanol.
(FR) L'invention concerne une souche recombinée de Saccharomyces cerevisiae comprenant une voie xylose-éthanol et exprimant au moins un acide nucléique exogène codant pour un transporteur de xylose, une xylose réductase, une xylose déshydrogénase, et une xylulokinase provenant d'une souche de Metschnikowia déposée auprès de l'Autorité de dépôt internationale du Canada (IDAC) sous le numéro d'enregistrement 081116-01, et comprenant en outre une transaldolase et une délétion de PH013, et un procédé de production d'éthanol.
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