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1. (WO2018107879) SILVER STAINING KIT FOR DETECTING DNA IN POLYACRYLAMIDE GEL, AND USE THEREOF

Pub. No.:    WO/2018/107879    International Application No.:    PCT/CN2017/105452
Publication Date: Fri Jun 22 01:59:59 CEST 2018 International Filing Date: Wed Oct 11 01:59:59 CEST 2017
IPC: G01N 1/30
Applicants: GUANGZHOU UNIVERSITY
广州大学
Inventors: GUO, Peiguo
郭培国
LI, Ronghua
李荣华
LIU, Wenjie
刘文杰
XIA, Yanshi
夏岩石
Title: SILVER STAINING KIT FOR DETECTING DNA IN POLYACRYLAMIDE GEL, AND USE THEREOF
Abstract:
Provided is a silver staining kit for detecting DNA in a polyacrylamide gel, the kit comprising reagent A, wherein the reagent is an aqueous solution, and in each litre of the aqueous solution, there is 3-5 mL of glacial acetic acid, 1-3 g of silver nitrate and 50-100 mL of ethanol; reagent B, wherein the reagent is an aqueous solution, and in each litre of the aqueous solution, there is 0.5-1 g of sodium chloride, 0.5-2.5 g of sodium sulphite, 0.1-0.3 g of carbohydrazide, 2-10 g of sodium carbonate and 5-10 g of glucose; and reagent C, wherein the reagent is an aqueous solution, and in each litre of the aqueous solution, there is 3-5 mL of glacial acetic acid.