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1. (WO2018107376) PURIFICATION METHOD FOR GINSENOSIDE RH2

Pub. No.:    WO/2018/107376    International Application No.:    PCT/CN2016/109848
Publication Date: Fri Jun 22 01:59:59 CEST 2018 International Filing Date: Thu Dec 15 00:59:59 CET 2016
IPC: B01D 11/04
A61K 36/258
C12P 19/44
A61K 31/704
A61P 35/00
Applicants: BONTAC BIO-ENGINEERING (SHENZHEN) CO.,LTD
邦泰生物工程(深圳)有限公司
JIANGXI BONTAC GREEN-BIOCATALYSIS ECOINDUSTRIAL PARK CO.,LTD.
江西邦泰绿色生物合成生态产业园发展有限公司
Inventors: FU, Rongzhao
傅荣昭
LIU, Lihui
刘立辉
YAN, Xin
鄢欣
Title: PURIFICATION METHOD FOR GINSENOSIDE RH2
Abstract:
A purification method for ginsenoside Rh2, used for purifying an enzyme reaction liquid that prepares Rh2 by catalyzing Rg3 with β-glucosidase, aims at solving the technical problem in the existing purification method of complex operation, time and labor consuming and higher cost. The purification method comprises: extracting the enzyme reaction liquid with an extraction system; standing and layering same, and then preserving an upper solution; and performing solvent recovery on the upper solution to obtain the product Rh2. The extraction system consists of ammonium sulfate, ethanol, and ethyl acetate; the dosage of ammonium sulfate is 2-20 g/100 ml of the enzyme reaction liquid; the overall volume of ethanol and ethyl acetate is 0.5-5 times the volume of the enzyme reaction liquid.