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|1. (WO2018107376) PURIFICATION METHOD FOR GINSENOSIDE RH2|
|Applicants:||BONTAC BIO-ENGINEERING (SHENZHEN) CO.,LTD
JIANGXI BONTAC GREEN-BIOCATALYSIS ECOINDUSTRIAL PARK CO.,LTD.
|Title:||PURIFICATION METHOD FOR GINSENOSIDE RH2|
A purification method for ginsenoside Rh2, used for purifying an enzyme reaction liquid that prepares Rh2 by catalyzing Rg3 with β-glucosidase, aims at solving the technical problem in the existing purification method of complex operation, time and labor consuming and higher cost. The purification method comprises: extracting the enzyme reaction liquid with an extraction system; standing and layering same, and then preserving an upper solution; and performing solvent recovery on the upper solution to obtain the product Rh2. The extraction system consists of ammonium sulfate, ethanol, and ethyl acetate; the dosage of ammonium sulfate is 2-20 g/100 ml of the enzyme reaction liquid; the overall volume of ethanol and ethyl acetate is 0.5-5 times the volume of the enzyme reaction liquid.