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1. (WO2018106536) METHODS FOR MAKING AND USING DEDIFFERENTIATED AND STEM-LIKE HUMAN CELLS

Pub. No.:    WO/2018/106536    International Application No.:    PCT/US2017/064250
Publication Date: Fri Jun 15 01:59:59 CEST 2018 International Filing Date: Sat Dec 02 00:59:59 CET 2017
IPC: A61K 35/12
A61K 39/395
A61L 27/36
C07K 16/28
C12N 5/00
Applicants: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventors: WAWRZYNIAK, Joseph
CHERESH, David
Title: METHODS FOR MAKING AND USING DEDIFFERENTIATED AND STEM-LIKE HUMAN CELLS
Abstract:
Provided are methods for making highly dedifferentiated and stem-like human cells from human umbilical vein endothelial cells (HUVECs) ectopically expressing integrin β3. Also provided are methods for making ectoderm, mesoderm, and endoderm cells from HUVECs ectopically expressing integrin β3. Also provided are methods for making neural cells, or cells having neuronal-like morphology, from HUVECs ectopically expressing integrin β3. Provided are methods for making cardiomyocytes, or cells having cardiomyocyte-like morphology, from HUVECs ectopically expressing integrin β3. Provided are methods for the production of pluripotent stem cells comprising expressing integrin β3 in primary human endothelial cells. In alternative embodiments, provided are methods for inducing αvβ3 clustering, and to accelerate or facilitate angiogenesis, tissue remodeling or repair, or wound healing, for example, to accelerate healing after an infarction.