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1. (WO2018104962) HEMIAMINAL-TAG FOR PROTEIN LABELING AND PURIFICATION
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Pub. No.: WO/2018/104962 International Application No.: PCT/IN2017/050570
Publication Date: 14.06.2018 International Filing Date: 05.12.2017
IPC:
C07K 5/00 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
5
Peptides having up to four amino acids in a fully defined sequence; Derivatives thereof
Applicants:
INDIAN INSTITUTE OF SCIENCE EDUCATION AND RESEARCH BHOPAL [IN/IN]; Bhopal Bypass Road, Bhauri Bhopal, Madhya Pradesh 462066, IN
Inventors:
RAI, Vishal; IN
PURUSHOTTAM, Landa; IN
Agent:
KUMAR, G. Arun; IN
YAMUNA, A.; IN
KUMARI, Jyoti; IN
PANDEY, Shachindra Kumar; IN
SINGH, Ranjana; IN
SINGH, Shikha; IN
Priority Data:
20162104180807.12.2016IN
Title (EN) HEMIAMINAL-TAG FOR PROTEIN LABELING AND PURIFICATION
(FR) ÉTIQUETTE HÉMIAMINAL POUR LE MARQUAGE ET LA PURIFICATION DE PROTÉINES
Abstract:
(EN) The invention pertains to the synthesis, isolation, and characterization of hemiaminal for selective labeling of peptides, proteins, antibodies, and organic fragments with -C(=0) CH2NH2 and derivatives with -CH2NH2 group over -C(=0) CHRNH2 group (where R≠H). The invention also pertains to the method of single-site immobilization of proteins through N-terminus Gly on solid phase. The invention includes late-stage tagging of N-terminus Gly with an affinity tag, 19F NMR probe, and a fluorophore and a method for metal-free protein purification and isolation of analytically pure proteins.
(FR) L'invention concerne la synthèse, l'isolement et la caractérisation d'hémiaminal pour le marquage sélectif de peptides, de protéines, d'anticorps et de fragments organiques avec -C(=0) CH2NH2 et des dérivés ayant un groupe -CH2NH2 sur le groupe -C(=0) CHRNH2 (où R ≠ H). L'invention concerne également le procédé d'immobilisation mono-site de protéines par l'intermédiaire d'une phase solide sur l'extrémité N-terminale Gly. L'invention comprend le marquage au stade tardif de l'extrémité N-terminale Gly avec une étiquette d'affinité, une sonde 19F RMN, et un fluorophore, ainsi qu'un procédé de purification de protéines exempts de métaux et l'isolement de protéines pures de manière analytique.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)