Search International and National Patent Collections

1. (WO2018104908) LINKED LIGATION

Pub. No.:    WO/2018/104908    International Application No.:    PCT/IB2017/057732
Publication Date: Fri Jun 15 01:59:59 CEST 2018 International Filing Date: Fri Dec 08 00:59:59 CET 2017
IPC: C12Q 1/6855
C12N 15/09
C12Q 1/686
C12Q 1/6869
Applicants: BOREAL GENOMICS, INC.
Inventors: MARZIALI, Andrea
PEL, Joel
Title: LINKED LIGATION
Abstract:
The invention generally relates to capturing, amplifying, and sequencing nucleic acids. In certain embodiments, copies of the sense and antisense strands of a duplex template nucleic acid are captured using linked capture probes and multiple binding and extension steps to improve specificity over traditional single binding target capture techniques. Methods of seeding sequencing clusters with sense and antisense strands of a target nucleic acid are also disclosed including identifying the strands using sense-specific barcodes and confirming base calls using two sense-specific sequencing reads. Linked adapters may be used to increase adapter ligation selectively or efficiency and yield.