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1. (WO2018104301) APPARATUS FOR CHARACTERIZING LUMINESCENT ENTITIES

Pub. No.:    WO/2018/104301    International Application No.:    PCT/EP2017/081526
Publication Date: Fri Jun 15 01:59:59 CEST 2018 International Filing Date: Wed Dec 06 00:59:59 CET 2017
IPC: G02B 21/00
G01N 21/64
G02B 27/56
C12Q 1/68
G02B 21/16
Applicants: GNOTHIS AB
Inventors: RIGLER, Rudolf
EDMAN, Lars
RÖNNLUND, Daniel
TIIMAN, Margo
Title: APPARATUS FOR CHARACTERIZING LUMINESCENT ENTITIES
Abstract:
An apparatus for characterizing luminescent entities by excitation comprising:• a substrate (6) being in contact with a solution comprising luminescent entities; • a source of electromagnetic radiation (4) providing at least a primary beam of radiation (8);an objective (5); a first optical element (1) capable of transforming the intensity profile of the primary beam (8) into an arbitrary secondary intensity profile (distribution) (9); a second optical element (2) capable of separating (discriminating) radiation by wavelength; and a detector (7), where the arbitrary secondary intensity profile has at least an off-center circular continuous intensity distribution (33) focused on the back focal plane (12) of the objective forming a collimated beam (10) capable of creating an evanescent field on the side of the substrate where the solution comprising luminescent entities are located, where the evanescent field excites the luminescent entities thereby creating emission radiation separated by the second optical element (2) and captioned by the detector (7). The invention also relates to an apparatus comprising two optical elements providing a final third intensity profile (distribution) which is the convolution of two mathematical transformations corresponding to each of optical element one and four, respectively.