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1. (WO2018104282) METHOD FOR PRODUCING TRANSPARENT PREPARATIONS FOR EXAMINATION BY LIGHT MICROSCOPY

Pub. No.:    WO/2018/104282    International Application No.:    PCT/EP2017/081476
Publication Date: Fri Jun 15 01:59:59 CEST 2018 International Filing Date: Wed Dec 06 00:59:59 CET 2017
IPC: G01N 1/30
G01N 33/483
G02B 21/34
G01N 27/447
Applicants: GEORG-AUGUST-UNIVERSITÄT GÖTTINGEN STIFTUNG ÖFFENTLICHEN RECHTS, UNIVERSITÄTSMEDIZIN
Inventors: VENTZKI, Robert
WOUTERS-BUNT, Fred S.
Title: METHOD FOR PRODUCING TRANSPARENT PREPARATIONS FOR EXAMINATION BY LIGHT MICROSCOPY
Abstract:
The invention relates to a method for producing transparent biological preparations for examination by light microscopy, wherein biological tissue is electrophoretically clarified in that the tissue is immersed in an aqueous alkaline electrophoresis solution and is exposed to an electric field in the electrophoresis solution. The electrophoresis solution contains a buffer base, the cations of which have a molecular weight of at least 50 Da, at a concentration of 5 to 100 mol/m3 and a non-ionic detergent at a concentration of 0.1 to 10% (w/v).