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1. (WO2018104238) KAURENOIC ACID HYDROXYLASES
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Pub. No.: WO/2018/104238 International Application No.: PCT/EP2017/081390
Publication Date: 14.06.2018 International Filing Date: 04.12.2017
IPC:
C12N 9/02 (2006.01) ,A23L 27/30 (2016.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02
Oxidoreductases (1.), e.g. luciferase
[IPC code unknown for A23L 27/30]
Applicants:
DSM IP ASSETS B.V. [NL/NL]; Het Overloon 1 6411 TE Heerlen, NL
Inventors:
VAN LEEUWEN, Johannes, Gustaaf, Ernst; NL
ZWARTJENS, Priscilla; NL
BOER, Viktor, Marius; NL
Agent:
DSM INTELLECTUAL PROPERTY; P.O. Box 4 6100 AA Echt, NL
Priority Data:
16202945.808.12.2016EP
Title (EN) KAURENOIC ACID HYDROXYLASES
(FR) HYDROXYLASES D'ACIDE KAURÉNOÏQUE
Abstract:
(EN) The present invention relates a polypeptide having kaurenoic acid 13-hydroxylase activity, which polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1 or SEQ ID NO: 3, comprises at least one substitution of an amino acid corresponding to any of amino acids at positions 136, 248, 336 or 403, said positions being defined with reference to SEQ ID NO: 1 or SEQ ID NO: 3 and wherein the polypeptide has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
(FR) La présente invention concerne un polypeptide possédant une activité de 13-hydroxylase d'acide kaurénoïque, ledit polypeptide comprenant une séquence d'acides aminés qui, lorsqu'elle est alignée avec une 13-hydroxylase d'acide kaurénoïque comprenant la séquence présentée dans SEQ ID NO : 1 ou SEQ ID NO : 3, comprend au moins une substitution d'un acide aminé correspondant à un acide aminé quelconque aux positions 136, 248, 336 ou 403, lesdites positions étant définies par référence à SEQ ID NO : 1 ou SEQ ID NO : 3, le polypeptide ayant une ou plusieurs propriétés modifiées par rapport à un polypeptide de référence ayant une activité de 13-hydroxylase d'acide kaurénoïque. Un polypeptide de l'invention peut être utilisé dans un hôte recombiné pour la production de stéviol ou d'un glycoside de stéviol.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)